Abstract
Two methods(A and B)of selecting effect on transgenic wheat from immature seed and the mature seed with different concentration of Kanamycin are studied.Results showed that under sterile condition,younger seedlings of non-transgenic wheat from immature seed(A) could be refrained effectively by Kanamycin of 50 mg/L and wheat plants from mature seed(B) could be refrained by kanamycin of 80～100 mg/L.Compared to B,A was more sensitive to Kanamycin,has shorter selecting period and the result was more credible confirmed by PCR analysis.Therefore,A was an efficient selection method for transgenic wheat with nptⅡ mark gene.
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Claims 6
Claim · model-written summary
Under sterile conditions, young non-transgenic wheat seedlings from immature seed (method A) were effectively inhibited by 50 mg/L kanamycin.
- Taxon
- wheat
Triticum aestivumt0 - Organ / stage
- younger seedlingse0
- Outcome
- refrained effectivelye0
- Value
- 50 mg/Le0
- Setting
- sterile conditione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“under sterile condition,younger seedlings of non-transgenic wheat from immature seed(A) could be refrained effectively by Kanamycin of 50 mg/L”
Claim · model-written summary
Wheat plants from mature seed (method B) were inhibited by 80-100 mg/L kanamycin.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- could be refrainede0
- Value
- 80～100 mg/Le0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“wheat plants from mature seed(B) could be refrained by kanamycin of 80～100 mg/L”
Claim · model-written summary
Method A was more sensitive to kanamycin than method B.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- more sensitive to Kanamycine0
- Comparator
- Be0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“Compared to B,A was more sensitive to Kanamycin”
Claim · model-written summary
Method A had a shorter selecting period than method B.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- shorter selecting periode0
- Comparator
- Be0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“Compared to B,A was more sensitive to Kanamycin,has shorter selecting period”
Claim · model-written summary
With method A the selection result was more credible, as confirmed by PCR analysis, than with method B.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- more credible confirmed by PCR analysise0
- Comparator
- Be0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“Compared to B,A was more sensitive to Kanamycin,has shorter selecting period and the result was more credible confirmed by PCR analysis”
Claim · model-written summary
The authors conclude that method A is an efficient selection method for transgenic wheat with the nptII marker gene.
- Taxon
- wheatTriticum aestivume0
- Outcome
- efficient selection methode0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A was an efficient selection method for transgenic wheat”