Abstract
A method for determination of sucrose was developed by spectrophotometry.It was found that in sulˉphuric acid medium sucrose can reduce phosphorus molybdenum yellowto form phosphorus molybdenum blue.The reaction was investigated in detail and the optimal reaction conditions were obtained.The maximum absorption wavelength of resulting phosphorus molybdenum blue is at820nm.Beer's law is obeyed over the concentration range of0.57～22.56mg/L of sucrose and the apparent molar absorbancy index is1.17×10 4 L/(mol·cm).The method was applied to the determination of sucrose content in sugarcane juice samples with satisfactory results.The mechanism of reaction of sucrose with phosphorus molybdenum yellowwas discussed.
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Claims 4
Claim · model-written summary
The reaction product had maximum absorption at 820 nm.
Verbatim
t0“Sugarcane Juice”
e0“The maximum absorption wavelength of resulting phosphorus molybdenum blue is at820nm”
Claim · model-written summary
The method obeyed Beer’s law from 0.57 to 22.56 mg/L sucrose.
Verbatim
t0“Sugarcane Juice”
e0“Beer's law is obeyed over the concentration range of0.57～22.56mg/L of sucrose”
Claim · model-written summary
The apparent molar absorbancy index was 1.17×10 4 L/(mol·cm).
Verbatim
t0“Sugarcane Juice”
e0“the apparent molar absorbancy index is1.17×10 4 L/(mol·cm)”
Claim · model-written summary
The assay determined sucrose in sugarcane juice with satisfactory results.
Not reportedComparator
Verbatim
e0“The method was applied to the determination of sucrose content in sugarcane juice samples with satisfactory results”