Abstract
DNA was extracted from seeds, root tips, hypocotyls and leaflets of peanut using the CTAB method and SDS method, respectively. The results showed that the MW of the DNA samples was higher than 21 kb,and that using the same method, the purity of the DNAs extracted from different parts was not different, but the DNA production rate significantly differed, with leaflets producing the largest amount and seeds producing the smallest amount. The DNA production rate varied from 128.5 to 498.5 ng/mg with the CTAB method, and ranged from 225.0 to 542.6 ng/mg with the SDS method. Between the extraction methods, the DNA production rate of the same tissue type significantly differed, and as far as the DNA purity of the same tissue type was concerned, only leaflets significantly differed. RAPD analysis among 19 peanut cultivars using DNAs extracted from leaflets with the CTAB method indicated that DNAs were up to the standard for molecular analysis and RAPD could be used to identify the cultivars of peanut.
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Claims 9
Claim · model-written summary
the MW of the DNA samples was higher than 21 kb.
- Taxon
- Peanut
Arachis hypogaeat0 - Outcome
- MW of the DNA samplese0
- Direction
- highere0
- Value
- 21 kbe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Peanut”
e0“the MW of the DNA samples was higher than 21 kb”
Claim · model-written summary
using the same method, the purity of the DNAs extracted from different parts was not different.
- Taxon
- PeanutArachis hypogaeat0
- Outcome
- purity of the DNAse0
- Direction
- not differente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Peanut”
e0“using the same method, the purity of the DNAs extracted from different parts was not different”
Claim · model-written summary
the DNA production rate significantly differed, with leaflets producing the largest amount and seeds producing the smallest amount.
- Taxon
- PeanutArachis hypogaeat0
- Organ / stage
- leafletse0
- Outcome
- DNA production ratee0
- Direction
- largeste0
- Comparator
- seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Peanut”
e0“the DNA production rate significantly differed, with leaflets producing the largest amount and seeds producing the smallest amount”
Claim · model-written summary
The DNA production rate varied from 128.5 to 498.5 ng/mg with the CTAB method.
- Taxon
- PeanutArachis hypogaeat0
- Treatment
- CTAB methode0
- Outcome
- DNA production ratee0
- Value
- 128.5 to 498.5 ng/mge0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Peanut”
e0“The DNA production rate varied from 128.5 to 498.5 ng/mg with the CTAB method”
Claim · model-written summary
ranged from 225.0 to 542.6 ng/mg with the SDS method.
- Taxon
- PeanutArachis hypogaeat0
- Treatment
- SDS methode0
- Outcome
- DNA production ratee1
- Value
- 225.0 to 542.6 ng/mge0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Peanut”
e0“ranged from 225.0 to 542.6 ng/mg with the SDS method”
e1“The DNA production rate varied from 128.5 to 498.5 ng/mg with the CTAB method”
Claim · model-written summary
Between the extraction methods, the DNA production rate of the same tissue type significantly differed.
- Taxon
- PeanutArachis hypogaeat0
- Outcome
- DNA production ratee0
- Comparator
- extraction methodse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Peanut”
e0“Between the extraction methods, the DNA production rate of the same tissue type significantly differed”
Claim · model-written summary
only leaflets significantly differed.
- Taxon
- PeanutArachis hypogaeat0
- Organ / stage
- leafletse0
- Outcome
- leafletse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Peanut”
e0“only leaflets significantly differed”
Claim · model-written summary
DNAs were up to the standard for molecular analysis.
- Taxon
- PeanutArachis hypogaeat0
- Outcome
- DNAs were up to the standard for molecular analysise0
- Value
- 19 peanut cultivarse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Peanut”
e0“DNAs were up to the standard for molecular analysis”
e1“19 peanut cultivars”
Claim · model-written summary
RAPD could be used to identify the cultivars of peanut.
- Taxon
- PeanutArachis hypogaeat0
- Outcome
- identify the cultivars of peanute0
- Value
- 19 peanut cultivarse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Peanut”
e0“RAPD could be used to identify the cultivars of peanut”
e1“19 peanut cultivars”