Abstract
A seed-specific expression gene (f128) was isolated from Setaria italica cDNA library. Sequence analysis indicated that the f128 gene was composed of 581 nt which was deduced containing an open reading frame coding for a 9.1 kD polypeptide of 85 amino acid residues(Accession No. of f128 in genBank:AY327511). The results of analysis by Biology Software showed that in the structure of the deduced protein the hydrophobic domain was dominant. It seems to have a cleavable N-terminal signal sequence, and it may entry to a secretory pathway. Genomic Southern blotting analysis indicated that f128 was one copy in genome of the millet. Northern blotting analysis showed that f128 mRNA only was accumulated in developing seeds.
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Claims 6
Claim · model-written summary
f128 mRNA accumulated only in developing seeds.
- Taxon
- Setaria italica
Setaria italicae1 - Organ / stage
- developing seedse6
- Gene
- f128e6
- Outcome
- f128 mRNAe6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A seed-specific expression gene (f128) was isolated from Setaria italica cDNA library”
e6“Northern blotting analysis showed that f128 mRNA only was accumulated in developing seeds”
Claim · model-written summary
The f128 gene contains an open reading frame coding for a 9.1 kD polypeptide of 85 amino acid residues.
- Taxon
- Setaria italicaSetaria italicae1
- Gene
- f128 genee2
- Outcome
- open reading frame coding for a 9.1 kD polypeptidee2
- Value
- 9.1 kDe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A seed-specific expression gene (f128) was isolated from Setaria italica cDNA library”
e2“the f128 gene was composed of 581 nt which was deduced containing an open reading frame coding for a 9.1 kD polypeptide of 85 amino acid residues”
Claim · model-written summary
f128 is present as one copy in the millet genome.
- Taxon
- Setaria italicaSetaria italicae1
- Gene
- f128e5
- Outcome
- genome of the millete5
- Value
- one copye5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A seed-specific expression gene (f128) was isolated from Setaria italica cDNA library”
e5“Genomic Southern blotting analysis indicated that f128 was one copy in genome of the millet”
Claim · model-written summary
The deduced f128 protein has a dominant hydrophobic domain.
- Taxon
- Setaria italicaSetaria italicae1
- Outcome
- hydrophobic domaine3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A seed-specific expression gene (f128) was isolated from Setaria italica cDNA library”
e3“in the structure of the deduced protein the hydrophobic domain was dominant”
Claim · model-written summary
The deduced f128 protein seems to have a cleavable N-terminal signal sequence.
- Taxon
- Setaria italicaSetaria italicae1
- Outcome
- cleavable N-terminal signal sequencee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A seed-specific expression gene (f128) was isolated from Setaria italica cDNA library”
e4“It seems to have a cleavable N-terminal signal sequence, and it may entry to a secretory pathway”
Claim · model-written summary
The f128 protein may enter a secretory pathway.
- Taxon
- Setaria italicaSetaria italicae1
- Outcome
- secretory pathwaye4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A seed-specific expression gene (f128) was isolated from Setaria italica cDNA library”
e4“It seems to have a cleavable N-terminal signal sequence, and it may entry to a secretory pathway”