Abstract
Three ARF10 gene(sAuxin-Respond-Factor)named CsARF10a,CsARF10b and CsARF10c, were screened from the cucumber genome database by protein homology BLAST and cloned from 8419s-1 by polymerase chain reaction(PCR). In present study,phylogenetic reconstruction indicated that the CsARF10a has closer relation with SlARF10,while CsARF10b and CsARF10c have higher similarity with AtARF10. Promoter prediction showed that the promoters consist of diversified multiple hormone response elements. The real-time quantitative PCR(RT-qPCR)analysis proved that the expression of cucumber ARF10 genes could be regulated by different phytohormones or concentrations of 1-naphthylacetic acid (NAA)and performed different expression patterns. It was also seen that CsARF10 gene subfamily had high transcriptional levels either in roots,stems,leaves or in male flowers;In additional,the qPCR results showed that although the expression of CsARF10 genes have similar transcriptional trend,the CsARF10a had high expression level while mRNA of CsARF10b and CsARF10c were relatively low.
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Claims 10
Claim · model-written summary
Three CsARF10 genes were identified and cloned from 8419s-1.
- Taxon
- Cucumber
Cucumis sativust0 - Material
- 8419s-1e0
- Gene
- CsARF10a,CsARF10b and CsARF10ce0
- Outcome
- screened from the cucumber genome databasee0
- Value
- Three ARF10 genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cucumber”
e0“Three ARF10 gene(sAuxin-Respond-Factor)named CsARF10a,CsARF10b and CsARF10c, were screened from the cucumber genome database by protein homology BLAST and cloned from 8419s-1”
Claim · model-written summary
CsARF10a is more closely related to SlARF10.
- Taxon
- CucumberCucumis sativust0
- Gene
- CsARF10ae0
- Outcome
- closer relation with SlARF10e0
- Comparator
- SlARF10e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cucumber”
e0“the CsARF10a has closer relation with SlARF10”
Claim · model-written summary
CsARF10b and CsARF10c have higher similarity with AtARF10.
- Taxon
- CucumberCucumis sativust0
- Gene
- CsARF10b and CsARF10ce0
- Outcome
- higher similarity with AtARF10e0
- Comparator
- AtARF10e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cucumber”
e0“CsARF10b and CsARF10c have higher similarity with AtARF10”
Claim · model-written summary
Their promoters contain diverse hormone response elements.
- Taxon
- CucumberCucumis sativust0
- Outcome
- diversified multiple hormone response elementse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cucumber”
e0“the promoters consist of diversified multiple hormone response elements”
Claim · model-written summary
Different phytohormones regulated cucumber ARF10 expression in different patterns.
- Taxon
- CucumberCucumis sativust0
- Treatment
- different phytohormonese0
- Gene
- cucumber ARF10 genese0
- Outcome
- expression of cucumber ARF10 genese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cucumber”
e0“the expression of cucumber ARF10 genes could be regulated by different phytohormones or concentrations of 1-naphthylacetic acid (NAA)and performed different expression patterns”
Claim · model-written summary
Different NAA concentrations regulated cucumber ARF10 expression in different patterns.
- Taxon
- CucumberCucumis sativust0
- Treatment
- concentrations of 1-naphthylacetic acid (NAA)e0
- Gene
- cucumber ARF10 genese0
- Outcome
- expression of cucumber ARF10 genese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cucumber”
e0“the expression of cucumber ARF10 genes could be regulated by different phytohormones or concentrations of 1-naphthylacetic acid (NAA)and performed different expression patterns”
Claim · model-written summary
CsARF10 genes had high transcription in roots, stems, leaves and male flowers.
- Taxon
- CucumberCucumis sativust0
- Organ / stage
- roots,stems,leaves or in male flowerse0
- Gene
- CsARF10 gene subfamilye0
- Outcome
- high transcriptional levelse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cucumber”
e0“CsARF10 gene subfamily had high transcriptional levels either in roots,stems,leaves or in male flowers”
Claim · model-written summary
CsARF10 genes had similar transcriptional trends.
- Taxon
- CucumberCucumis sativust0
- Gene
- CsARF10 genese0
- Outcome
- similar transcriptional trende0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cucumber”
e0“the expression of CsARF10 genes have similar transcriptional trend”
Claim · model-written summary
CsARF10a expression was high.
- Taxon
- CucumberCucumis sativust0
- Gene
- CsARF10ae0
- Outcome
- high expression levele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cucumber”
e0“the CsARF10a had high expression level”
Claim · model-written summary
CsARF10b and CsARF10c mRNA levels were relatively low.
- Taxon
- CucumberCucumis sativust0
- Gene
- CsARF10b and CsARF10ce0
- Outcome
- mRNA of CsARF10b and CsARF10ce0
- Direction
- relatively lowe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cucumber”
e0“mRNA of CsARF10b and CsARF10c were relatively low”