Abstract
Objective:To develop an effective method for total RNA isolation from the root tissue of Ophiopogon japonicus(L.F.) Ker-Gawl rich in polysaccharides and polyphenolics.Methods:Improved phenol method was applied.RNA extraction buffer was composed of 5% SDS,1 mol /L NaAc(pH4.1),20% HAC,and 0.1% PVP.Results:The RNA extraction protocol presented might be used to efficiently isolate high quality RNA with high purity and integrity,clear electrophoresis.Yield and purity were monitored by gel electrophoresis and by UV absorbance.D260nm/D280nm and D260nm/D230nm absorbance ratio of root tissue RNA of O.japonicus were higher than 1.8 and 2.0 respectively.Average yields of RNA of touberous root and adventitious root were about 79.716 and 76.144 μg /g(FW) respectively.Conclusion:The RNA extracted by this method was of sufficient quality for use in SSH reaction.
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Claims 6
Claim · model-written summary
The protocol efficiently isolated high quality, pure, intact root RNA.
- Taxon
- Ophiopogon japonicus(L.F.) Ker-Gawl
Ophiopogon japonicust0 - Outcome
- high quality RNA with high purity and integritye0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The RNA extraction protocol presented might be used to efficiently isolate high quality RNA with high purity and integrity,clear electrophoresis”
t0“Ophiopogon japonicus (L.F.) Ker-Gawl”
Claim · model-written summary
Root RNA D260nm/D280nm absorbance ratio exceeded 1.8.
- Taxon
- O.japonicusOphiopogon japonicuse0
- Outcome
- D260nm/D280nme0
- Direction
- highere0
- Value
- 1.8e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“D260nm/D280nm and D260nm/D230nm absorbance ratio of root tissue RNA of O.japonicus were higher than 1.8 and 2.0 respectively”
Claim · model-written summary
Root RNA D260nm/D230nm absorbance ratio exceeded 2.0.
- Taxon
- O.japonicusOphiopogon japonicuse0
- Outcome
- D260nm/D230nme0
- Direction
- highere0
- Value
- 2.0e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“D260nm/D280nm and D260nm/D230nm absorbance ratio of root tissue RNA of O.japonicus were higher than 1.8 and 2.0 respectively”
Claim · model-written summary
Tuberous-root RNA yield averaged about 79.716 μg /g(FW).
- Taxon
- Ophiopogon japonicus (L.F.) Ker-GawlOphiopogon japonicust0
- Organ / stage
- touberous roote0
- Outcome
- yields of RNA of touberous roote0
- Value
- 79.716e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Average yields of RNA of touberous root and adventitious root were about 79.716 and 76.144 μg /g(FW) respectively”
t0“Ophiopogon japonicus (L.F.) Ker-Gawl”
Claim · model-written summary
Adventitious-root RNA yield averaged about 76.144 μg /g(FW).
- Taxon
- Ophiopogon japonicus (L.F.) Ker-GawlOphiopogon japonicust0
- Organ / stage
- adventitious roote0
- Outcome
- yields of RNA of touberous root and adventitious roote0
- Value
- 76.144 μg /g(FW)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Average yields of RNA of touberous root and adventitious root were about 79.716 and 76.144 μg /g(FW) respectively”
t0“Ophiopogon japonicus (L.F.) Ker-Gawl”
Claim · model-written summary
The extracted RNA was sufficiently high quality for SSH.
- Taxon
- Ophiopogon japonicus (L.F.) Ker-GawlOphiopogon japonicust0
- Outcome
- sufficient quality for use in SSH reactione0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The RNA extracted by this method was of sufficient quality for use in SSH reaction”
t0“Ophiopogon japonicus (L.F.) Ker-Gawl”