Abstract
Taking uredospores of Cronartium ribicola,the Pinus armandii bark infected byCronartium ribicola and the healthy bark as experimental materials,the differences in metabolites extracted with methanol were analyzed by means of UV scanning,TLC and HPLC.The results indicated that the metabolite extracted from uredospores of Cronartium ribicola had two strong absorptive peaks at 214 nm and 307 nm wave lengths,but there were no evident peak values absorbed by the metabolite of tree barks.It was showed by UV scanning at 307 nm wave length that there was a component commonly detected in metabolites extracted from both the infected bark and the uredospores,which was not found out in the metabolite extracted from the healthy bark.Solvent system(ethyl acetate ∶ethanol=15 ∶1)could differentiate the metabolites extracted from the infected and the healthy barks of Pinus armandii.HPLC detected results also showed that the metabolites extracted from both the uredospores of Cronartium ribicola and the infected bark had similar absorption peak at 214 nm wave length while the retaining time was from 10.30 min to 10.40 min,but the metabolite extracted from the healthy bark did not have this special peak.The above resulted showed the possibility of telling the difference of the infected plants from the healthy ones.
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Claims 6
Claim · model-written summary
Uredospore metabolites showed two strong absorption peaks.
- Taxon
- Cronartium ribicola
Cronartium ribicolae0 - Outcome
- two strong absorptive peakse0
- Value
- 214 nm and 307 nme0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the metabolite extracted from uredospores of Cronartium ribicola had two strong absorptive peaks at 214 nm and 307 nm wave lengths”
Claim · model-written summary
Bark metabolites showed no evident absorption peaks.
- Outcome
- no evident peak valuese0
Not reportedTaxonDirection
Verbatim
e0“there were no evident peak values absorbed by the metabolite of tree barks.”
Claim · model-written summary
At 307 nm, a component was shared by infected bark and uredospores but absent from healthy bark.
Not reportedTaxonDirection
Verbatim
e0“there was a component commonly detected in metabolites extracted from both the infected bark and the uredospores,which was not found out in the metabolite extracted from the healthy bark.”
Claim · model-written summary
An ethyl acetate–ethanol solvent system distinguished infected from healthy Pinus armandii bark metabolites.
- Taxon
- Pinus armandii
Pinus armandiie0 - Treatment
- ethyl acetate ∶ethanol=15 ∶1e0
- Outcome
- differentiate the metabolitese0
- Comparator
- infected and the healthy barkse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Solvent system(ethyl acetate ∶ethanol=15 ∶1)could differentiate the metabolites extracted from the infected and the healthy barks of Pinus armandii.”
Claim · model-written summary
HPLC found a shared 214 nm peak in uredospores and infected bark, absent in healthy bark.
- Outcome
- similar absorption peake0
- Value
- 214 nme0
- Qualifier
- 10.30 min to 10.40 mine0
- Comparator
- healthy barke0
Not reportedTaxon
Verbatim
e0“the metabolites extracted from both the uredospores of Cronartium ribicola and the infected bark had similar absorption peak at 214 nm wave length while the retaining time was from 10.30 min to 10.40 min,but the metabolite extracted from the healthy bark did not have this special peak.”
Claim · model-written summary
The metabolite tests may distinguish infected from healthy plants.
Not reportedTaxon
Verbatim
e0“the possibility of telling the difference of the infected plants from the healthy ones.”