Abstract
Objective:To establish the qualitative and quantitative analysis method for the total flavonols in the flowers of Rosa rugosa.Methods:To detect quercetin and kaempferol by silica TLC with toluene - ethyl formateformic acid(10:8:1) as developing solvent and 3%aluminium trichloride alcoholic solution as visualization reagent; To determine the content of quercetin and kaempferol by HPLC,with C_(18) column Phenomenex Luna(250 mm×4.6 mm,5μm) and mobile phase methanol -0.4%phosphoric acid(60:40),at a flow rate of 1 mL·min~(-1) and detective wavelength 360 nm for assay.Results:Quercetin and kaempferol were detected by silica gel TLC.By HPLC,the linear range of quercetin was 0.06 - 0.6μg with a correlation coefficient 0.9996,the average recovery (n =5) was 99.2%with RSD of 3.9%;the linear range of kaempferol was 0.02 - 0.2μg with a correlation coefficient 0.9993,the average recovery(n =5) was 98.5%with RSD of 4.8%.Conclusion:Qualitative and quantitative analysis method for the total flavonols in the flowers of Rosa rugosa has been built up for the first time,the results also provide a basis for the quality control of Rosa rugosa.
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Claims 6
Claim · model-written summary
TLC detected quercetin in Rosa flowers.
- Taxon
- Rosa rugosa
Rosa rugosat0 - Outcome
- Quercetine0
- Comparator
- silica gel TLCe0
Not reportedValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Quercetin and kaempferol were detected by silica gel TLC.”
t0“flowers of Rosa rugosa”
Claim · model-written summary
TLC detected kaempferol in Rosa flowers.
- Taxon
- Rosa rugosaRosa rugosat0
- Outcome
- kaempferole0
- Comparator
- silica gel TLCe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Quercetin and kaempferol were detected by silica gel TLC.”
t0“flowers of Rosa rugosa”
Claim · model-written summary
Quercetin HPLC linear range was 0.06 - 0.6μg with correlation coefficient 0.9996.
- Taxon
- Rosa rugosaRosa rugosat0
- Outcome
- linear range of quercetine0
- Value
- 0.06 - 0.6μge0
- Qualifier
- correlation coefficient 0.9996e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the linear range of quercetin was 0.06 - 0.6μg with a correlation coefficient 0.9996”
t0“flowers of Rosa rugosa”
Claim · model-written summary
Quercetin recovery averaged 99.2%, with 3.9% RSD.
- Taxon
- Rosa rugosaRosa rugosat0
- Outcome
- average recoverye0
- Value
- 99.2%e0
- Qualifier
- RSD of 3.9%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the average recovery (n =5) was 99.2%with RSD of 3.9%”
e1“quercetin was 0.06 - 0.6μg”
t0“flowers of Rosa rugosa”
Claim · model-written summary
Kaempferol HPLC linear range was 0.02 - 0.2μg with correlation coefficient 0.9993.
- Taxon
- Rosa rugosaRosa rugosat0
- Outcome
- linear range of kaempferole0
- Value
- 0.02 - 0.2μge0
- Qualifier
- correlation coefficient 0.9993e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the linear range of kaempferol was 0.02 - 0.2μg with a correlation coefficient 0.9993”
t0“flowers of Rosa rugosa”
Claim · model-written summary
Kaempferol recovery averaged 98.5%, with 4.8% RSD.
- Taxon
- Rosa rugosaRosa rugosat0
- Outcome
- average recoverye0
- Value
- 98.5%e0
- Qualifier
- RSD of 4.8%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the average recovery(n =5) was 98.5%with RSD of 4.8%”
e1“kaempferol was 0.02 - 0.2μg”
t0“flowers of Rosa rugosa”