Abstract
Rice stripe virus encoded Pc2 protein is involved in the viral infection and symptom formation of rice.Study on the interaction between Pc2 and host is essential to elucidate the molecular mechanism of viral infection.In this study,the encoding region of Pc2-N or Pc2-C was fused to the pGBKT7,the rice cDNA library was constructed and screened by the Pc2-N or Pc2-C as a bait to identify proteins that interacted with Pc2.The results showed that the coding regions and the amino acid sequences of insert Pc2-N and Pc2-C were correct.Pc2-N had neither toxicity nor self activating effect on AH109,while Pc2-C was toxic to AH109.The cDNA library had a titer o f 4×107 CFU/mL,and most of insert fragments were between 500 bp to 2 000 bp in size.Eight candidate positive clones were obtained by screening with Pc2-N as a bait.Their cDNA Sequences were obtained and BLAST results showed that these cDNAs encoded 5 proteins,which mainly were the proteins involved in photosystem and stress.
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Claims 5
Claim · model-written summary
Pc2-N was neither toxic nor self-activating in AH109, whereas Pc2-C was toxic.
Not reportedDirection
Verbatim
e0“Pc2-N had neither toxicity nor self activating effect on AH109,while Pc2-C was toxic to AH109.”
Claim · model-written summary
The rice cDNA library had a titer of 4×107 CFU/mL.
Verbatim
e0“The cDNA library had a titer o f 4×107 CFU/mL,and most of insert fragments were between 500 bp to 2 000 bp in size.”
Claim · model-written summary
Most library insert fragments were 500 to 2,000 bp long.
Verbatim
e0“The cDNA library had a titer o f 4×107 CFU/mL,and most of insert fragments were between 500 bp to 2 000 bp in size.”
Claim · model-written summary
Screening with Pc2-N yielded eight candidate positive clones.
Verbatim
e0“Eight candidate positive clones were obtained by screening with Pc2-N as a bait.”
Claim · model-written summary
The candidate cDNAs encoded five proteins, mainly involved in photosystems and stress.
- Taxon
- Rice
Oryza sativat0 - Outcome
- proteins involved in photosystem and stresse0
- Value
- 5 proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Their cDNA Sequences were obtained and BLAST results showed that these cDNAs encoded 5 proteins,which mainly were the proteins involved in photosystem and stress.”
t0“Rice Stripe Virus”