Abstract
A capillary electrophoresis(CE)with UV detection method was developed for the determination of ellagic acid and punicalagin in pomegranate peel,grown in different origins.Effects of some important factors,such as pH value,concentration of running buffer,separation voltage and injection time were investigated.The optimum conditions were as the follows:running buffer:50 mmol/L borax buffer(pH 8.7),separation voltage:20 kV,injection time:9 s/20 kV.Under the optimum conditions,two analytes could be separated within 12 min at the detection wavelength of 260 nm.The good linearities between peak area and mass concentration were obtained in the ranges of 5.0×10-7-5.0×10-5 g/mL and 2.0×10-6-5.0×10-5 g/mL for ellagic acid and punicalagin,with their detection limits of 1.0×10-7g/mL and 1.3×10-6g/mL,respectively.The relative standard deviations(RSD,n=7) for the peak areas of ellagic acid and punicalagin were 2.4% and 2.2%,respectively.The average recoveries were 101% for ellagic acid and 98% for punicalagin.It is found by t-test that there were no significant differences between this method and the HPLC method.The proposed method was simple,rapid and accurate,and was successfully applied in the determination of the analytes in pomegranate peel samples.
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Claims 10
Claim · model-written summary
The method separated both analytes within 12 min.
Verbatim
e0“two analytes could be separated within 12 min at the detection wavelength of 260 nm”
Claim · model-written summary
Ellagic acid had a linear range of 5.0×10-7-5.0×10-5 g/mL.
Verbatim
e0“5.0×10-7-5.0×10-5 g/mL and 2.0×10-6-5.0×10-5 g/mL for ellagic acid and punicalagin”
Claim · model-written summary
Punicalagin had a linear range of 2.0×10-6-5.0×10-5 g/mL.
Verbatim
e0“2.0×10-6-5.0×10-5 g/mL for ellagic acid and punicalagin”
Claim · model-written summary
Ellagic acid detection limit was 1.0×10-7g/mL.
Verbatim
e0“detection limits of 1.0×10-7g/mL and 1.3×10-6g/mL”
Claim · model-written summary
Punicalagin detection limit was 1.3×10-6g/mL.
Verbatim
e0“detection limits of 1.0×10-7g/mL and 1.3×10-6g/mL”
Claim · model-written summary
Ellagic acid peak-area RSD was 2.4%.
Verbatim
e0“peak areas of ellagic acid and punicalagin were 2.4% and 2.2%”
Claim · model-written summary
Punicalagin peak-area RSD was 2.2%.
Verbatim
e0“peak areas of ellagic acid and punicalagin were 2.4% and 2.2%”
Claim · model-written summary
Average ellagic acid recovery was 101%.
Verbatim
e0“average recoveries were 101% for ellagic acid and 98% for punicalagin”
Claim · model-written summary
Average punicalagin recovery was 98%.
Verbatim
e0“98% for punicalagin”
Claim · model-written summary
The method did not significantly differ from HPLC.
Verbatim
e0“there were no significant differences between this method and the HPLC method”