Paper · 2002

A rapid immunological determination method for metalaxyl residue in plant products

Hong Zhou; C Zhengjiang

8
claims
8
verbatim quotes
0
citations
1
research areas
—
Not open access

Abstract

Metalaxyl was acidified to its acid and the synthetized antigens were prepared by coupling the acid to carrying protein with the method of mixed anhydride and were used as immunogenes to immunize the rabbits.The titer of the antisera measured with indirect ELISA was1∶ 204 800. The determination method for metalaxyl was set up.The range of detection was 1 953 ng/mL to 250 0 ng/mL,which was fit for the demand of quantitative determination of Metalaxyl in plant products.The coincidance rate between the test and the gas chromatography was 92%.The average recovery of fortified rice,wheat and tomato was 97%,97 47%and 99 45%respectively.Cross reactivity indicated that the antisera could only react with metalaxyl and its acid,but not with those matters whose structures are similar to metalaxyl including metolachlor,diethatyl ethyl,alachlor propachlor and benzoylprop ethyl.This method is simple,rapid and sensitive and fit for determination of metalaxyl residue in plant products.

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Claims 8

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

The metalaxyl antisera reached an indirect ELISA titer of 1∶ 204 800.

Outcome
titer of the antiserae1
Value
1∶ 204 800e1

Not reportedTaxon

Verbatim

e1

“The titer of the antisera measured with indirect ELISA was1∶ 204 800”

✓ located verbatim in the source · abstract, characters 208–276 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

The metalaxyl ELISA had a detection range of about 1953 to 2500 ng/mL.

Outcome
range of detectione2
Value
1 953 ng/mL to 250 0 ng/mLe2

Not reportedTaxon

Verbatim

e2

“The range of detection was 1 953 ng/mL to 250 0 ng/mL”

✓ located verbatim in the source · abstract, characters 328–381 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

The ELISA agreed with gas chromatography at a coincidence rate of 92%.

Outcome
coincidance ratee3
Value
92%e3
Comparator
gas chromatographye3

Not reportedTaxon

Verbatim

e3

“The coincidance rate between the test and the gas chromatography was 92%”

✓ located verbatim in the source · abstract, characters 472–544 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Average recovery of metalaxyl from fortified rice was 97%.

Outcome
average recoverye4
Value
97%e4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e4

“The average recovery of fortified rice,wheat and tomato was 97%,97 47%and 99 45%respectively”

✓ located verbatim in the source · abstract, characters 545–637 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Average recovery of metalaxyl from fortified wheat was 97.47%.

Outcome
average recoverye4
Value
97 47%e4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e4

“The average recovery of fortified rice,wheat and tomato was 97%,97 47%and 99 45%respectively”

✓ located verbatim in the source · abstract, characters 545–637 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Average recovery of metalaxyl from fortified tomato was 99.45%.

Outcome
average recoverye4
Value
99 45%e4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e4

“The average recovery of fortified rice,wheat and tomato was 97%,97 47%and 99 45%respectively”

✓ located verbatim in the source · abstract, characters 545–637 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

The antisera cross-reacted only with metalaxyl and its acid, not with the listed structurally similar compounds.

Outcome
Cross reactivitye5
Comparator
metolachlor,diethatyl ethyl,alachlor propachlor and benzoylprop ethyle5

Not reportedTaxon

Verbatim

e5

“Cross reactivity indicated that the antisera could only react with metalaxyl and its acid,but not with those matters whose structures are similar to metalaxyl including metolachlor,diethatyl ethyl,alachlor propachlor and benzoylprop ethyl”

✓ located verbatim in the source · abstract, characters 638–876 · show in text ↑
Observationauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

The method is simple, rapid and sensitive.

Outcome
simple,rapid and sensitivee6

Not reportedTaxon

Verbatim

e6

“This method is simple,rapid and sensitive and fit for determination of metalaxyl residue in plant products”

✓ located verbatim in the source · abstract, characters 877–983 · show in text ↑

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