Abstract
Sucrose synthase 2 (SuSy2) is a key enzyme regulating sucrose metabolism in higher plants, which promotes sucrose into various metabolic pathways. The degenerated oligonucleotides to highly conserved region of SuSy2 were used to prime the amplification of specific fragment by PCR in samples of sugarcane DNA. One fragment of about 7.5 kb was cloned and its complete nucleotide sequence was determined. Its nucleotide sequence and the protein from its coding region was 99.5% and 100% identical to the deduced proteins from the sugarcane SuSy2 cDNA. The sequence also included about 1.9 kb upstream promoter regulation,sixteen extrons, fifteen introns, 3' untranslatded region, and the open read frame coded 802 amino acids. The aminoacid sequence had a conservative sucrose metabolism key enzyme family Ser/Thr bit point.All that laid the foundation for its transcriptional expression regulation mechanism.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
A roughly 7.5 kb sugarcane SuSy2 fragment was cloned and sequenced.
Verbatim
e0“One fragment of about 7.5 kb was cloned and its complete nucleotide sequence was determined.”
t0“Sugarcane(Saccharum officenarum L.)”
Claim · model-written summary
The cloned nucleotide sequence was 99.5% identical to sugarcane SuSy2 cDNA.
Verbatim
e0“Its nucleotide sequence and the protein from its coding region was 99.5% and 100% identical to the deduced proteins from the sugarcane SuSy2 cDNA.”
Claim · model-written summary
The coding region protein was reported as 100% identical to the sugarcane SuSy2 cDNA-derived protein.
Verbatim
e0“Its nucleotide sequence and the protein from its coding region was 99.5% and 100% identical to the deduced proteins from the sugarcane SuSy2 cDNA.”
Claim · model-written summary
The sugarcane SuSy2 sequence contained a promoter region, exons, introns and a 3' untranslated region.
Verbatim
e0“The sequence also included about 1.9 kb upstream promoter regulation,sixteen extrons, fifteen introns, 3' untranslatded region”
t0“Sugarcane(Saccharum officenarum L.)”
Claim · model-written summary
The open reading frame encoded 802 amino acids.
Verbatim
e0“the open read frame coded 802 amino acids”
t0“Sugarcane(Saccharum officenarum L.)”
Claim · model-written summary
The amino acid sequence had a conserved sucrose metabolism enzyme-family Ser/Thr site.
Verbatim
e0“The aminoacid sequence had a conservative sucrose metabolism key enzyme family Ser/Thr bit point.”
t0“Sugarcane(Saccharum officenarum L.)”