Paper · 2013 · Chinese Journal of Spectroscopy Laboratory

Determination of Polysaccharides of Osmanthus fragrans Lour. by Fluorescence Spectrometry

Lin Zhi

8
claims
16
verbatim quotes
0
citations
3
research areas
—
Not open access

Abstract

The content of polysaccharides inOsmanthus fragransLour.was determined by fluorescence spectrometry with anhydrous glucose as standard.The optimal experimental conditions were as follows:Concentrated sulfuric acid of 5.00mL,6% phenol solution of 1.00mL,reaction time of 10 min and fluorescence parameters atKex=478nm andKem=510nm.Polysaccharides ofOsmanthus fragransLour. were analyzed under the optimal experimental conditions. The concentration of polysaccharide had a good linear relationship with its fluorescence intensity.The linear equation was y=1.0776x-4.1145,r=0.9991,and the detection limit was 2.50Lg/mL.

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Claims 8

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Optimal assay conditions used 5.00mL concentrated sulfuric acid.

Taxon
Osmanthus fragransOsmanthus fragranse1
Outcome
optimal experimental conditionse0
Value
5.00mLe0
Qualifier
Concentrated sulfuric acide0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Osmanthus fragrans
Osmanthus fragrans · TaxonLaitr Keiows · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The optimal experimental conditions were as follows:Concentrated sulfuric acid of 5.00mL,6% phenol solution of 1.00mL,reaction time of 10 min and fluorescence parameters atKex=478nm andKem=510nm”

✓ located verbatim in the source · abstract, characters 135–329 · show in text ↑
e1

“inOsmanthus fragransLour.”

✓ located verbatim in the source · abstract, characters 31–56 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Optimal assay conditions used 1.00mL of 6% phenol solution.

Taxon
Osmanthus fragransOsmanthus fragranse1
Outcome
optimal experimental conditionse0
Value
1.00mLe0
Qualifier
6% phenol solutione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Osmanthus fragrans
Osmanthus fragrans · TaxonLaitr Keiows · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The optimal experimental conditions were as follows:Concentrated sulfuric acid of 5.00mL,6% phenol solution of 1.00mL,reaction time of 10 min and fluorescence parameters atKex=478nm andKem=510nm”

✓ located verbatim in the source · abstract, characters 135–329 · show in text ↑
e1

“inOsmanthus fragransLour.”

✓ located verbatim in the source · abstract, characters 31–56 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Optimal reaction time was 10 min.

Taxon
Osmanthus fragransOsmanthus fragranse1
Outcome
reaction timee0
Value
10 mine0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Osmanthus fragrans
Osmanthus fragrans · TaxonLaitr Keiows · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“reaction time of 10 min”

✓ located verbatim in the source · abstract, characters 253–276 · show in text ↑
e1

“inOsmanthus fragransLour.”

✓ located verbatim in the source · abstract, characters 31–56 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Optimal excitation wavelength was 478nm.

Taxon
Osmanthus fragransOsmanthus fragranse1
Outcome
Kexe0
Value
478nme0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Osmanthus fragrans
Osmanthus fragrans · TaxonLaitr Keiows · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“fluorescence parameters atKex=478nm andKem=510nm”

✓ located verbatim in the source · abstract, characters 281–329 · show in text ↑
e1

“inOsmanthus fragransLour.”

✓ located verbatim in the source · abstract, characters 31–56 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Optimal emission wavelength was 510nm.

Taxon
Osmanthus fragransOsmanthus fragranse1
Outcome
Keme0
Value
510nme0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Osmanthus fragrans
Osmanthus fragrans · TaxonLaitr Keiows · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“fluorescence parameters atKex=478nm andKem=510nm”

✓ located verbatim in the source · abstract, characters 281–329 · show in text ↑
e1

“inOsmanthus fragransLour.”

✓ located verbatim in the source · abstract, characters 31–56 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Polysaccharide concentration had a good linear relationship with fluorescence intensity.

Taxon
Osmanthus fragransOsmanthus fragranse1
Compound
polysaccharidee0
Outcome
fluorescence intensitye0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Osmanthus fragrans
Osmanthus fragrans · TaxonLaitr Keiows · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The concentration of polysaccharide had a good linear relationship with its fluorescence intensity”

✓ located verbatim in the source · abstract, characters 429–527 · show in text ↑
e1

“inOsmanthus fragransLour.”

✓ located verbatim in the source · abstract, characters 31–56 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

The calibration equation was y=1.0776x-4.1145 with r=0.9991.

Taxon
Osmanthus fragransOsmanthus fragranse1
Outcome
linear equatione0
Value
r=0.9991e0
Qualifier
y=1.0776x-4.1145e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Osmanthus fragrans
Osmanthus fragrans · TaxonLaitr Keiows · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The linear equation was y=1.0776x-4.1145,r=0.9991”

✓ located verbatim in the source · abstract, characters 528–577 · show in text ↑
e1

“inOsmanthus fragransLour.”

✓ located verbatim in the source · abstract, characters 31–56 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

The assay detection limit was 2.50Lg/mL.

Taxon
Osmanthus fragransOsmanthus fragranse1
Outcome
detection limite0
Value
2.50Lg/mLe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Osmanthus fragrans
Osmanthus fragrans · TaxonLaitr Keiows · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the detection limit was 2.50Lg/mL”

✓ located verbatim in the source · abstract, characters 582–615 · show in text ↑
e1

“inOsmanthus fragransLour.”

✓ located verbatim in the source · abstract, characters 31–56 · show in text ↑