Paper · 2008 · Acta Pratacultural Science

Evaluation of universality and efficacy of microsatellite markers amplified in Elymus taxa from China

Yuxia Guo

6
claims
10
verbatim quotes
1
citations
2
research areas
—
Not open access

Abstract

Microsatellite markers from related species are commonly used in plant genetics,but much unpredictability exists.Different sources of microsatellite markers were amplified in nine Elymus species in China.Results indicated that six Elymus and five Triticum aestivum markers had stable PCR amplification and gave better genetic analytical analysis.Loci ECGA22、ECGA114、EAGA51 and WMS43 were highly informative and helpful in construction of high-resolution genetic fingerprints of Elymus taxa from China.There were few differences in allele numbers between this research and that in former publications using higher genetic indexes and similar gene flow to the materials used in this study.This suggests that more homologous primers are even more efficient.

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Claims 6

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Six Elymus markers amplified stably and gave better genetic analysis.

Taxon
ElymusElymuse0
Outcome
stable PCR amplificatione0
Value
six Elymuse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Elymus
Elymus · Taxon · picture of the genus (one of its species)Public domain · Wikimedia Commons ↗

Verbatim

e0

“six Elymus and five Triticum aestivum markers had stable PCR amplification and gave better genetic analytical analysis”

✓ located verbatim in the source · abstract, characters 227–345 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Five Triticum aestivum markers amplified stably and gave better genetic analysis.

Taxon
ElymusElymust0
Outcome
stable PCR amplificatione0
Value
five Triticum aestivume0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Elymus
Elymus · Taxon · picture of the genus (one of its species)Public domain · Wikimedia Commons ↗

Verbatim

t0

“Elymus taxa”

✓ located verbatim in the source · title, characters 79–90 · show in text ↑
e0

“six Elymus and five Triticum aestivum markers had stable PCR amplification and gave better genetic analytical analysis”

✓ located verbatim in the source · abstract, characters 227–345 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

ECGA22, ECGA114 and EAGA51 were highly informative for Elymus fingerprints.

Taxon
ElymusElymust0
Gene
ECGA22、ECGA114、EAGA51e0
Outcome
highly informativee0
Value
high-resolutione0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Elymus
Elymus · Taxon · picture of the genus (one of its species)Public domain · Wikimedia Commons ↗

Verbatim

t0

“Elymus taxa”

✓ located verbatim in the source · title, characters 79–90 · show in text ↑
e0

“Loci ECGA22、ECGA114、EAGA51 and WMS43 were highly informative and helpful in construction of high-resolution genetic fingerprints of Elymus taxa from China”

✓ located verbatim in the source · abstract, characters 346–500 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

WMS43 was highly informative for Elymus fingerprints.

Taxon
ElymusElymuse0
Gene
WMS43e0
Outcome
highly informativee0
Value
high-resolutione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Elymus
Elymus · Taxon · picture of the genus (one of its species)Public domain · Wikimedia Commons ↗

Verbatim

e0

“Loci ECGA22、ECGA114、EAGA51 and WMS43 were highly informative and helpful in construction of high-resolution genetic fingerprints of Elymus taxa from China”

✓ located verbatim in the source · abstract, characters 346–500 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Allele numbers differed little from earlier publications.

Taxon
ElymusElymust0
Outcome
allele numberse0
Direction
few differencese0
Comparator
former publicationse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Elymus
Elymus · Taxon · picture of the genus (one of its species)Public domain · Wikimedia Commons ↗

Verbatim

t0

“Elymus taxa”

✓ located verbatim in the source · title, characters 79–90 · show in text ↑
e0

“There were few differences in allele numbers between this research and that in former publications”

✓ located verbatim in the source · abstract, characters 501–599 · show in text ↑
Method / resourceauthor interpretationoriginal resultmeasured

Claim · model-written summary

More homologous primers were inferred to be more efficient.

Taxon
ElymusElymust0
Outcome
more efficiente0

Not reportedComparatorValue

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Elymus
Elymus · Taxon · picture of the genus (one of its species)Public domain · Wikimedia Commons ↗

Verbatim

t0

“Elymus taxa”

✓ located verbatim in the source · title, characters 79–90 · show in text ↑
e0

“more homologous primers are even more efficient”

✓ located verbatim in the source · abstract, characters 706–753 · show in text ↑