Abstract
Pratylenchus brachyurus was isolated from the brown necrotic roots of Guava(Psidium guajava)in Xunxiao,Zhangzhou,Fujian Province.By applying light microscope and scanning electronic microscope,the nematode's diagnostic characters are as follows: 2 lips annule,labial disc oblong and surrounded by 6 lips including 2 lateral lips and 4 subrounded submedial lips;amphidial apertures narrow oval,closely abutting both sides of lip disc;stylet 15.3~19.9 μm,basal knobs round;ovary straight,spermatheca inconspicuous,post uterine sac slightly shorter than width at vulva,vulva situated at 83%~86%of body length;tail broadly conoid with ventral annules 16~21,tip smooth and broadly rounded.The rDNA-ITS and 28S rDNA-D2D3 of the nematode were amplified and analyzed,the sequences were 730 bp and 779~780 bp respectively and their similarity with the corresponding sequences of P.brachyurus in Genbank were 89%~93%and 97%~99%respectively.Using D2D3 was more suitable than using ITS in the molecular identification of P.brachyurus.The results of isolation and staining of the nematodes within the diseased root tissues confirmed that the damaged Guava's roots were infected by P.brachyurus inducing black rotten roots and brown lesions on the root cortex.It was the first report in China that P.brachyurus was isolated in the root of Guava.
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Claims 7
Claim · model-written summary
Pratylenchus brachyurus was isolated from necrotic Guava roots in Fujian Province.
- Taxon
- Guava
Psidium guajavae0 - Organ / stage
- rootse0
- Interactor
- Pratylenchus brachyurusPratylenchus brachyuruse0pathogen
- Outcome
- Pratylenchus brachyurus was isolatede0
- Setting
- Xunxiao,Zhangzhou,Fujian Provincee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pratylenchus brachyurus was isolated from the brown necrotic roots of Guava(Psidium guajava)in Xunxiao,Zhangzhou,Fujian Province”
Claim · model-written summary
The nematode showed the reported diagnostic morphology.
- Taxon
- GuavaPsidium guajavae0
- Outcome
- the nematode's diagnostic characterse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“roots of Guava(Psidium guajava)”
e1“the nematode's diagnostic characters are as follows: 2 lips annule,labial disc oblong and surrounded by 6 lips including 2 lateral lips and 4 subrounded submedial lips;amphidial apertures narrow oval,closely abutting both sides of lip disc;stylet 15.3~19.9 μm,basal knobs round;ovary straight,spermatheca inconspicuous,post uterine sac slightly shorter than width at vulva,vulva situated at 83%~86%of body length;tail broadly conoid with ventral annules 16~21,tip smooth and broadly rounded”
Claim · model-written summary
The nematode ITS sequence was 730 bp and 89%~93% similar to reference P. brachyurus.
- Taxon
- GuavaPsidium guajavae0
- Interactor
- P.brachyurusPratylenchus brachyuruse1pathogen
- Outcome
- rDNA-ITSe1
- Value
- 730 bpe1
- Qualifier
- 89%~93%e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“roots of Guava(Psidium guajava)”
e1“The rDNA-ITS and 28S rDNA-D2D3 of the nematode were amplified and analyzed,the sequences were 730 bp and 779~780 bp respectively and their similarity with the corresponding sequences of P.brachyurus in Genbank were 89%~93%and 97%~99%respectively”
Claim · model-written summary
The nematode D2D3 sequence was 779~780 bp and 97%~99% similar to reference P. brachyurus.
- Taxon
- GuavaPsidium guajavae0
- Interactor
- P.brachyurusPratylenchus brachyuruse1pathogen
- Outcome
- 28S rDNA-D2D3e1
- Value
- 779~780 bpe1
- Qualifier
- 97%~99%e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“roots of Guava(Psidium guajava)”
e1“The rDNA-ITS and 28S rDNA-D2D3 of the nematode were amplified and analyzed,the sequences were 730 bp and 779~780 bp respectively and their similarity with the corresponding sequences of P.brachyurus in Genbank were 89%~93%and 97%~99%respectively”
Claim · model-written summary
D2D3 was more suitable than ITS for molecular identification of the Guava nematode.
- Taxon
- GuavaPsidium guajavae0
- Interactor
- P.brachyurusPratylenchus brachyuruse1pathogen
- Outcome
- molecular identification of P.brachyuruse1
- Direction
- more suitablee1
- Comparator
- ITSe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“root of Guava”
e1“Using D2D3 was more suitable than using ITS in the molecular identification of P.brachyurus”
Claim · model-written summary
Root staining confirmed P. brachyurus infection and associated black rot and cortical lesions in Guava.
- Taxon
- GuavaPsidium guajavae0
- Organ / stage
- rootse0
- Interactor
- P.brachyurusPratylenchus brachyuruse0pathogen
- Outcome
- black rotten roots and brown lesions on the root cortexe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results of isolation and staining of the nematodes within the diseased root tissues confirmed that the damaged Guava's roots were infected by P.brachyurus inducing black rotten roots and brown lesions on the root cortex”
Claim · model-written summary
The study reported the first isolation of P. brachyurus from Guava roots in China.
- Taxon
- GuavaPsidium guajavae0
- Interactor
- P.brachyurusPratylenchus brachyuruse0pathogen
- Outcome
- P.brachyurus was isolated in the roote0
- Setting
- Chinae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It was the first report in China that P.brachyurus was isolated in the root of Guava”