Abstract
A method based on high-performance anion exchange chromatography and pulsed ampere detector(HPAEC-PAD) for the determination of trehalose content in transgenic rice plants acclimation to the environmental stress was employed.Trehalose was extracted in boiling water and centrifuged,and the supernatant filtered through a 0.45 μm filter unit.Quantitative carbohydrate analysis is carried out by HPAEC with a Carbo-Pak PA1 column using the DX500 HPAEC system,with which the eluent is 0.125 mol·L-1 NaOH,a gold electrode is used as working electrode and Ag-AgCl electrode as reference electrode.Experimental results show that the linear relationship and repeatability are good.And the results are satisfactory.
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Claims 3
Claim · model-written summary
The chromatography method showed good linearity.
- Taxon
- transgenic rice plants
Oryza sativae1 - Outcome
- linear relationshipe0
- Value
- goode0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Experimental results show that the linear relationship and repeatability are good”
e1“determination of trehalose content in transgenic rice plants”
Claim · model-written summary
The chromatography method showed good repeatability.
- Taxon
- transgenic rice plantsOryza sativae1
- Outcome
- repeatabilitye0
- Value
- goode0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Experimental results show that the linear relationship and repeatability are good”
e1“determination of trehalose content in transgenic rice plants”
Claim · model-written summary
The authors judged the analytical results satisfactory.
- Taxon
- transgenic rice plantsOryza sativae1
- Outcome
- results are satisfactorye0
- Value
- satisfactorye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“And the results are satisfactory”
e1“determination of trehalose content in transgenic rice plants”