Paper · 2005 · HortScience

Rooting Osha Crown Cuttings Using IBA and NAA

Bennett J. Sondeno; Karen L. Panter

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Open access

Abstract

Osha ( Ligusticum porteri ) is a Rocky Mountain native frequently used as a medicinal herb. It is currently harvested largely from the wild. Studies have been under way since 2001 to find ways to propagate and produce the plant. To potentially increase rooting success of crown cuttings of osha, two different rooting hormones were used, each at two concentrations. Treatments were controls, 2500 ppm, and 5000 ppm solutions each of indole-3-butyric acid (IBA) and α-naphthalene acetic acid (NAA). Cuttings were soaked in deionized water or treatment solutions for 2 min. After soaking cuttings were stuck in sterilized sand in 725-mL 2 containers, one cutting per container. Containers were placed on a mist propagation bench at 21 °C in a completely randomized design under natural light and day lengths. Data taken were days to visible root and shoot, and presence or absence of root formation after 50 days. Results indicated only one of 70 cuttings (<1%) produced a shoot. Roots formed on 14% of control cuttings, 64% in 2500 ppm IBA, 86% in 5000 ppm IBA, 36% in 2500 ppm NAA, and 14% in 5000 ppm NAA. Days to rooting ranged from 14.9 (2500 ppm IBA) to 29.0 (5000 ppm NAA). Due to considerable variation in days to rooting, and the number of cuttings that did not root, analysis of variance showed no differences among treatments. Frequency analysis indicated differences among treatments in root presence or absence. The 2500 and 5000 ppm IBA treatments showed more root formation than the controls or either NAA treatment. This indicates IBA may enhance rooting of osha crown cuttings.

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Claims 10

Observationobservedoriginal resultmeasured

Claim · model-written summary

One of 70 osha cuttings produced a shoot.

Organ / stage
cuttingse0
Outcome
produced a shoote0
Value
<1%e0

Not reportedTaxon

Verbatim

e0

“Results indicated only one of 70 cuttings (<1%) produced a shoot.”

✓ located verbatim in the source · abstract, characters 912–977 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Control cuttings rooted at 14%.

Material
control cuttingse0
Outcome
Roots formede0
Value
14%e0

Not reportedTaxon

Verbatim

e0

“Roots formed on 14% of control cuttings”

✓ located verbatim in the source · abstract, characters 978–1017 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Rooting reached 64% with 2500 ppm IBA.

Treatment
2500 ppm IBAe0
Outcome
64%e0
Value
64%e0

Not reportedTaxonComparatorDirection

Verbatim

e0

“64% in 2500 ppm IBA”

✓ located verbatim in the source · abstract, characters 1019–1038 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Rooting reached 86% with 5000 ppm IBA.

Treatment
5000 ppm IBAe0
Outcome
86%e0
Value
86%e0

Not reportedTaxonComparatorDirection

Verbatim

e0

“86% in 5000 ppm IBA”

✓ located verbatim in the source · abstract, characters 1040–1059 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Rooting reached 36% with 2500 ppm NAA.

Treatment
2500 ppm NAAe0
Outcome
36%e0
Value
36%e0

Not reportedTaxonComparatorDirection

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e0

“36% in 2500 ppm NAA”

✓ located verbatim in the source · abstract, characters 1061–1080 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Rooting reached 14% with 5000 ppm NAA.

Treatment
5000 ppm NAAe0
Outcome
14%e0
Value
14%e0

Not reportedTaxonComparatorDirection

Verbatim

e0

“14% in 5000 ppm NAA.”

✓ located verbatim in the source · abstract, characters 1086–1106 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Days to rooting ranged from 14.9 with 2500 ppm IBA to 29.0 with 5000 ppm NAA.

Treatment
2500 ppm IBAe0
Outcome
Days to rootinge0
Value
14.9e0
Qualifier
29.0e0
Comparator
5000 ppm NAAe0

Not reportedTaxon

Verbatim

e0

“Days to rooting ranged from 14.9 (2500 ppm IBA) to 29.0 (5000 ppm NAA).”

✓ located verbatim in the source · abstract, characters 1107–1178 · show in text ↑
Observationobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Analysis of variance found no differences among treatments in days to rooting.

Outcome
no differences among treatmentse0
Direction
no differencese0

Not reportedTaxon

Verbatim

e0

“analysis of variance showed no differences among treatments.”

✓ located verbatim in the source · abstract, characters 1275–1335 · show in text ↑
Observationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Frequency analysis found treatment differences in root presence.

Outcome
differences among treatments in root presence or absencee0

Not reportedTaxon

Verbatim

e0

“Frequency analysis indicated differences among treatments in root presence or absence.”

✓ located verbatim in the source · abstract, characters 1336–1422 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Both IBA doses produced more rooting than controls or NAA.

Treatment
2500 and 5000 ppm IBA treatmentse0
Outcome
root formatione0
Direction
moree0
Comparator
the controls or either NAA treatmente0

Not reportedTaxon

Verbatim

e0

“The 2500 and 5000 ppm IBA treatments showed more root formation than the controls or either NAA treatment.”

✓ located verbatim in the source · abstract, characters 1423–1529 · show in text ↑