Abstract
We compared nucleotide sequences of exon 4 and part of exon 5 of alleles F and S of the Adh1 locus controlling alcohol dehydrogenase in sugar beet. The Adh1-F and Adh1-S sequences of the examined fragment were shown to differ by two nucleotides. Adenine (A) and cytosine (C) of Adh1-F were substituted by respectively thymine (T) and adenine (A) in Adh1-S. Consequently, glutamine and asparagine from the F subunit of ADH1 are replaced by valine and lysine, respectively. Because of differences in the amino acid content, the F subunit is by two elementary charges more negatively charged electrically than the S subunit, which correlates with differences in their electrophoretic mobility. Comparison of the examined Adh1 fragment of sugar beet with its counterparts in other plants showed that the sites bearing substitutions in the former species are classed as variable.
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Claims 8
Claim · model-written summary
Adh1-F and Adh1-S differed by two nucleotides in the examined fragment.
- Taxon
- sugar beet
Beta vulgaris subsp. vulgarist0 - Gene
- Adh1-F and Adh1-Se0
- Outcome
- differ by two nucleotidese0
- Value
- two nucleotidese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sugar beet”
e0“The Adh1-F and Adh1-S sequences of the examined fragment were shown to differ by two nucleotides.”
Claim · model-written summary
An adenine in Adh1-F was substituted by thymine in Adh1-S.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Gene
- Adh1-Fe0
- Outcome
- Adenine (A)e0
- Comparator
- thymine (T)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sugar beet”
e0“Adenine (A) and cytosine (C) of Adh1-F were substituted by respectively thymine (T) and adenine (A) in Adh1-S.”
Claim · model-written summary
A cytosine in Adh1-F was substituted by adenine in Adh1-S.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Gene
- Adh1-Fe0
- Outcome
- cytosine (C)e0
- Comparator
- adenine (A)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sugar beet”
e0“Adenine (A) and cytosine (C) of Adh1-F were substituted by respectively thymine (T) and adenine (A) in Adh1-S.”
Claim · model-written summary
Glutamine in the F subunit was replaced by valine.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Gene
- ADH1e0
- Outcome
- glutaminee0
- Comparator
- valinee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sugar beet”
e0“Consequently, glutamine and asparagine from the F subunit of ADH1 are replaced by valine and lysine, respectively.”
Claim · model-written summary
Asparagine in the F subunit was replaced by lysine.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Gene
- ADH1e0
- Outcome
- asparaginee0
- Comparator
- lysinee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sugar beet”
e0“Consequently, glutamine and asparagine from the F subunit of ADH1 are replaced by valine and lysine, respectively.”
Claim · model-written summary
The F subunit was two elementary charges more negative than the S subunit.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Gene
- F subunite0
- Outcome
- negatively charged electricallye0
- Direction
- more negativelye0
- Value
- two elementary chargese0
- Comparator
- S subunite0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sugar beet”
e0“Because of differences in the amino acid content, the F subunit is by two elementary charges more negatively charged electrically than the S subunit, which correlates with differences in their electrophoretic mobility.”
Claim · model-written summary
Charge differences correlated with electrophoretic mobility differences.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarist0
- Gene
- F subunite0
- Outcome
- electrophoretic mobilitye0
- Qualifier
- differences in the amino acid contente0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sugar beet”
e0“Because of differences in the amino acid content, the F subunit is by two elementary charges more negatively charged electrically than the S subunit, which correlates with differences in their electrophoretic mobility.”
Claim · model-written summary
Substitution sites in the sugar beet Adh1 fragment were variable across other plants.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarise0
- Gene
- Adh1e0
- Outcome
- sites bearing substitutionse0
- Qualifier
- classed as variablee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Comparison of the examined Adh1 fragment of sugar beet with its counterparts in other plants showed that the sites bearing substitutions in the former species are classed as variable.”