Abstract
The article is devoted to virus screening of wild plants of Ukraine’s flora. The object of the research is the Red Book plant Pulsatilla pratensis (L.) Mill., which grows on the territory of Kanev Nature Reserve. Isolated isometric infectious virus-like particles with diameters of 34, 36, 43, 47, 50 and 57 nm were isolated from selected plants of P. pratensis. In our research, determination of the infectious nature of the pathogen, host range, concentration of viruses in plants, species identity and virus isolation from the mixture in mixed viral infections were carried with using indicator plants. The typical viral symptoms were observed on indicator plants: browning of the leaf plate, mottling, chlorosis and necrosis. All symptoms were systemic and could be caused by a variety of viruse species. Virions with sizes from 34 to 43 nm produced the necrotic and chlorotic spotting on Chenopodium amaranticolor Coste and Reyn. On the other hand, virions with sizes from 47 to 57 nm produced the necrosis, chlorosis and deformation of the leaf plates on Cucumis sativus L. That is not typical for viruses previously discovered on P. pratensis. The viruses isolated in these plants viruses were cumulated in small concentrations and rapidly lost their infectivity. The number of isolated viruses was insufficient for their identification. Four bacteriophage isolates with long phage tails of different size were isolated from P. pratensis roots and radical soil. The biological (lytic) activity towards the tracer bacteria, the morphology of negative colonies, and bacteriophage protein structure were characterized. According to our research, it is possible to divide phages into three subgroups that probably correspond to three different types of viruses. Results of the polypeptide analysis may reflect an evolutionary process in a population of phages that had a common ancestor. Comparison of phage proteins of different hosts shows a variety of molecular weights of polypeptides comprising up to several dozens of proteins. The results of the current work lay the basis for studying the spread of viruses in nature and determination of their relationships in biocenoses.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 11
Claim · model-written summary
Infectious virus-like particles of six reported diameters were isolated from P. pratensis.
Verbatim
e0“Isolated isometric infectious virus-like particles with diameters of 34, 36, 43, 47, 50 and 57 nm were isolated from selected plants of P. pratensis.”
Claim · model-written summary
Indicator plants displayed systemic browning, mottling and chlorosis.
Verbatim
e0“The typical viral symptoms were observed on indicator plants: browning of the leaf plate, mottling, chlorosis and necrosis. All symptoms were systemic and could be caused by a variety of viruse species.”
Claim · model-written summary
Indicator plants displayed systemic necrosis.
Verbatim
e0“The typical viral symptoms were observed on indicator plants: browning of the leaf plate, mottling, chlorosis and necrosis. All symptoms were systemic and could be caused by a variety of viruse species.”
Claim · model-written summary
Virions of 34 to 43 nm produced necrotic and chlorotic spotting on Chenopodium amaranticolor.
- Taxon
- Chenopodium amaranticolor
Chenopodium amaranticolore0 - Treatment
- Virions with sizes from 34 to 43 nme0
- Interactor
- Virionse0pathogen
- Outcome
- necrotic and chlorotic spottinge0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Virions with sizes from 34 to 43 nm produced the necrotic and chlorotic spotting on Chenopodium amaranticolor Coste and Reyn.”
Claim · model-written summary
Virions of 47 to 57 nm produced necrosis, chlorosis and leaf deformation on Cucumis sativus.
- Taxon
- Cucumis sativus
Cucumis sativuse0 - Treatment
- virions with sizes from 47 to 57 nme0
- Interactor
- virionse0pathogen
- Outcome
- necrosis, chlorosis and deformation of the leaf platese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On the other hand, virions with sizes from 47 to 57 nm produced the necrosis, chlorosis and deformation of the leaf plates on Cucumis sativus L.”
Claim · model-written summary
Isolated viruses accumulated in small concentrations.
- Outcome
- small concentrationse0
Not reportedDirection
Verbatim
e0“The viruses isolated in these plants viruses were cumulated in small concentrations and rapidly lost their infectivity.”
Claim · model-written summary
Isolated viruses rapidly lost infectivity and were too few for identification.
Verbatim
e0“The viruses isolated in these plants viruses were cumulated in small concentrations and rapidly lost their infectivity. The number of isolated viruses was insufficient for their identification.”
Claim · model-written summary
Four bacteriophage isolates with long tails of different sizes were isolated from roots and adjacent soil.
- Taxon
- bacteriophageBacteriophage sp.e0
- Organ / stage
- rootse0
- Outcome
- isolates with long phage tails of different sizee0
- Value
- Foure0
Verbatim
e0“Four bacteriophage isolates with long phage tails of different size were isolated from P. pratensis roots and radical soil.”
Claim · model-written summary
The phages could be divided into three subgroups probably representing three virus types.
Verbatim
e0“According to our research, it is possible to divide phages into three subgroups that probably correspond to three different types of viruses.”
Claim · model-written summary
Polypeptide analysis may indicate evolution in a phage population sharing a common ancestor.
Verbatim
e0“Results of the polypeptide analysis may reflect an evolutionary process in a population of phages that had a common ancestor.”
Claim · model-written summary
Phage proteins from different hosts showed varied polypeptide molecular weights and up to several dozen proteins.
Verbatim
e0“Comparison of phage proteins of different hosts shows a variety of molecular weights of polypeptides comprising up to several dozens of proteins.”