Abstract
Interest and great demand for blueberry (Vaccinium corymbosum) have increased, as V. corymbosum is now one of the most economically important crops in Korea. It is expected that blueberry production and the area planted for cultivation will increase consistently in the years ahead because of high profitability and the consumer's demand for healthy ingredients. Effective mass production of blueberry is urgently needed for commercial cultivation establishment, but a main limitation is lack of a propagation system that produces a disease-free plant material for commercial plantation. A large amount of research has focused entirely on developing tissue culture techniques for blueberry propagation. However, controlling fungal and bacterial contamination of woody plant material is extremely difficult. Our study was conducted to investigate the effect of biocide addition during the in vitro culture of blueberry on plantlet growth and contamination occurrence. Four biocides, including Plant Preservative Mixture ($PPM^{TM}$), vancomycin, nystatin and penicillin G, were used in varying concentrations during the in vitro propagation of blueberry. When nystatin was added into the medium at low concentrations, the overall growth of blueberry plantlets was retarded. Addition of vancomycin and penicillin G in high concentrations decreased contamination but induced plantlet mortality. On the other hand, when 1ml/L $PPM^{TM}$ was added, the growth characteristics of blueberry plantlets did not significantly differ from non-treatment (control), and the contamination occurrence rate was very low. From these results, we found that the addition of the appropriate biocide could provide an effective method to reduce contamination in the culture process, thereby raising in vitro production efficiency.
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Claims 6
Claim · model-written summary
Nystatin added at low concentrations retarded the overall growth of blueberry plantlets.
- Taxon
- blueberrye1
- Treatment
- nystatin was added into the medium at low concentrationse1
- Outcome
- overall growth of blueberry plantletse1
- Direction
- retardede1
Not reportedComparator
Verbatim
e1“When nystatin was added into the medium at low concentrations, the overall growth of blueberry plantlets was retarded”
Claim · model-written summary
Vancomycin and penicillin G at high concentrations decreased contamination in blueberry in vitro culture.
- Taxon
- blueberryt0
- Treatment
- Addition of vancomycin and penicillin G in high concentrationse2
- Outcome
- contaminatione2
- Direction
- decreasede2
Not reportedComparator
Verbatim
t0“in vitro culture of blueberry”
e2“Addition of vancomycin and penicillin G in high concentrations decreased contamination but induced plantlet mortality”
Claim · model-written summary
Vancomycin and penicillin G at high concentrations induced plantlet mortality in blueberry.
- Taxon
- blueberryt0
- Treatment
- Addition of vancomycin and penicillin G in high concentrationse2
- Outcome
- plantlet mortalitye2
- Direction
- increaseinferrede2
Not reportedComparator
Verbatim
t0“in vitro culture of blueberry”
e2“Addition of vancomycin and penicillin G in high concentrations decreased contamination but induced plantlet mortality”
Claim · model-written summary
Adding 1 ml/L PPM did not significantly change blueberry plantlet growth relative to the control.
Verbatim
e3“when 1ml/L $PPM^{TM}$ was added, the growth characteristics of blueberry plantlets did not significantly differ from non-treatment (control)”
Claim · model-written summary
Adding 1 ml/L PPM gave a very low contamination occurrence rate in blueberry culture.
- Taxon
- blueberryt0
- Treatment
- 1ml/L $PPM^{TM}$e4
- Outcome
- contamination occurrence ratee5
- Direction
- lowerinferrede5
Not reportedComparator
Verbatim
t0“in vitro culture of blueberry”
e4“when 1ml/L $PPM^{TM}$ was added”
e5“the contamination occurrence rate was very low”
Claim · model-written summary
The authors recommend adding an appropriate biocide to reduce contamination in the blueberry in vitro culture process.
Verbatim
t0“in vitro culture of blueberry”
e6“the addition of the appropriate biocide could provide an effective method to reduce contamination in the culture process”
The same questions in other papers
in vitro · growth · VacciniumPositive and negative results
1 paper in the same direction as this paper · 10 in the opposite direction
- positive
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See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.