Abstract
A labelled DNA is synthesized in the etiolated wheat seedlings 72 hrs after a single addition of [2-14C]orotic acid (1 mCi per 250 seedlings). This DNA does not differ in the methylation degree of cytosine residues and base composition (radioactivity ratios 100.m5C/(C+m5C) and T/(C++m5C) from total seeding DNA. Thus, the radioactivity ratio 100.m5C/(C+m5C) represents in fact the methylation degree of cytosine residues in labelled DNA, and [2-14C]orotic acid can be used for the study of DNA methylation in higher plants. The newly synthesized DNA (21 hr incubation of seedlings with radioactive precursor) is methylated about 2 times less in comparison with fully synthesized radioactive DNA and total seedling DNA. This supports the assumption on a step-wise character of replicative DNA methylation in plants. Phenylacetic acid (30 mg/l) and 6-benzylaminopurine (l mg/l) do not influence the post-replicative DNA methylation, but auxin (FAA) very strongly inhibits the replicative DNA methylation. The phytohormone-induced changes in replicative DNA methylation are considered as a possible mechanism for regulation of DNA replication and, probably, of cell differentiation in higher plants.
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Claims 6
Claim · model-written summary
Newly labeled wheat seedling DNA had the same cytosine methylation and base composition as total seedling DNA.
- Taxon
- wheat seedling
Triticum aestivumt0 - Outcome
- methylation degree of cytosine residues and base compositione0
- Direction
- does not differe0
- Comparator
- total seeding DNAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This DNA does not differ in the methylation degree of cytosine residues and base composition (radioactivity ratios 100.m5C/(C+m5C) and T/(C++m5C) from total seeding DNA.”
t0“wheat seedling”
Claim · model-written summary
The radioactivity ratio represents methylation degree and labeled orotic acid can be used to study plant DNA methylation.
- Taxon
- higher plantsEmbryophytae0
- Treatment
- [2-14C]orotic acide0
- Outcome
- study of DNA methylatione0
- Value
- 100.m5C/(C+m5C)e0
Verbatim
e0“Thus, the radioactivity ratio 100.m5C/(C+m5C) represents in fact the methylation degree of cytosine residues in labelled DNA, and [2-14C]orotic acid can be used for the study of DNA methylation in higher plants.”
Claim · model-written summary
Newly synthesized wheat DNA was methylated about two times less than fully synthesized radioactive and total seedling DNA.
- Taxon
- wheat seedlingTriticum aestivumt0
- Outcome
- methylatede0
- Direction
- lesse0
- Value
- about 2 timese0
- Comparator
- fully synthesized radioactive DNA and total seedling DNAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The newly synthesized DNA (21 hr incubation of seedlings with radioactive precursor) is methylated about 2 times less in comparison with fully synthesized radioactive DNA and total seedling DNA.”
t0“wheat seedling”
Claim · model-written summary
Phenylacetic acid did not influence wheat post-replicative DNA methylation.
- Taxon
- wheat seedlingTriticum aestivumt0
- Treatment
- Phenylacetic acid (30 mg/l)e0
- Outcome
- post-replicative DNA methylatione0
- Direction
- do not influencee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phenylacetic acid (30 mg/l) and 6-benzylaminopurine (l mg/l) do not influence the post-replicative DNA methylation, but auxin (FAA) very strongly inhibits the replicative DNA methylation.”
t0“wheat seedling”
Claim · model-written summary
6-benzylaminopurine did not influence wheat post-replicative DNA methylation.
- Taxon
- wheat seedlingTriticum aestivumt0
- Treatment
- 6-benzylaminopurine (l mg/l)e0
- Outcome
- post-replicative DNA methylatione0
- Direction
- do not influencee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phenylacetic acid (30 mg/l) and 6-benzylaminopurine (l mg/l) do not influence the post-replicative DNA methylation, but auxin (FAA) very strongly inhibits the replicative DNA methylation.”
t0“wheat seedling”
Claim · model-written summary
Auxin strongly inhibited wheat replicative DNA methylation.
- Taxon
- wheat seedlingTriticum aestivumt0
- Treatment
- auxin (FAA)e0
- Outcome
- replicative DNA methylatione0
- Direction
- inhibitse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“auxin (FAA) very strongly inhibits the replicative DNA methylation.”
t0“wheat seedling”
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