Abstract
BACKGROUND: There are various methods for the cryopreservation of plant material, with each biological specimen potentially requiring protocol optimization to maximize success. OBJECTIVE: The aim of this study is to compare droplet-vitrification, encapsulation-dehydration, and the cryo-plate method for cryopreservation of protocorms of the orchid Arundina graminifolia, using silica gel and drying beads as the desiccation materials. MATERIALS AND METHODS: The cryo-plate method included preculture of protocorms, developed from seeds, placed on aluminium cryo-plates and embedded in alginate gel. Cryo-plates were surface dried using sterile filter paper, placed in Petri dishes containing 50 g silica gel or 30 g drying beads in a laminar air-flow cabinet. Specimens on cryo-plates were dehydrated to 25 % moisture content, placed into 2 mL cryotubes and plunged directly into liquid nitrogen for 1 d. RESULTS: For cryopreservation, the cryo-plate method, involving dehydration with 30 g drying beads gave the highest regrowth (77 %), followed by the encapsulation-dehydration method with 30 g drying beads (64 % regrowth) and the droplet-vitrification method, following exposure to PVS2 solution for 20 min (33 % regrowth). CONCLUSIONS: Regrowth of cryopreserved protocorms using the cryo-plate method was rapid with the highest survival and regrowth.
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Claims 4
Claim · model-written summary
Cryo-plate dehydration with 30 g drying beads yielded 77 % regrowth, the highest among tested methods.
- Taxon
- Arundina graminifolia
Arundina graminifoliat0 - Treatment
- cryo-plate method, involving dehydration with 30 g drying beadse0
- Outcome
- regrowthe0
- Direction
- higheste0
- Value
- 77 %e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the cryo-plate method, involving dehydration with 30 g drying beads gave the highest regrowth (77 %)”
t0“Arundina graminifolia”
Claim · model-written summary
Encapsulation-dehydration with 30 g drying beads yielded 64 % regrowth.
- Taxon
- Arundina graminifoliaArundina graminifoliat0
- Treatment
- encapsulation-dehydration method with 30 g drying beadse0
- Outcome
- regrowthe0
- Value
- 64 %e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the encapsulation-dehydration method with 30 g drying beads (64 % regrowth)”
t0“Arundina graminifolia”
Claim · model-written summary
Droplet-vitrification after 20 min PVS2 exposure yielded 33 % regrowth.
- Taxon
- Arundina graminifoliaArundina graminifoliat0
- Treatment
- droplet-vitrification method, following exposure to PVS2 solution for 20 mine0
- Outcome
- regrowthe0
- Value
- 33 %e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the droplet-vitrification method, following exposure to PVS2 solution for 20 min (33 % regrowth)”
t0“Arundina graminifolia”
Claim · model-written summary
Regrowth using the cryo-plate method was rapid and had the highest survival and regrowth.
Not reportedComparator
Verbatim
e0“Regrowth of cryopreserved protocorms using the cryo-plate method was rapid with the highest survival and regrowth.”