Abstract
Characterization of an isoflavone-inducible locus closely linked to the common nod genes of Bradyrhizobium japonicum USDA110 led to the discovery of two open reading frames, designated nolY and nolZ. These open reading frames are preceded by a sequence with strong similarity to a consensus NodD-binding site (nod box). Studies utilizing a nolZ'-'lacZ fusion indicated that inducible expression is dependent upon both NodD1 and NodW, transcriptional regulators that are required for the expression of the common nodulation genes (e.g., nodYABC) of B. japonicum. A deletion mutation within nolY produced only slight defects in nodulation of soybeans, siratro, and cowpeas, but stronger defects were observed in nodulation of mung beans. An insertion mutation within nolZ showed no nodulation defects in the host plants tested. Competition assays for nodule occupancy in soybeans did not show any decrease in the competitiveness of a nolY mutant, nor did a nolY mutant show any detectable alteration in the production of lipooligosaccharide nodulation signals.
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Claims 7
Claim · model-written summary
The locus contained two open reading frames, nolY and nolZ.
- Taxon
- soybeans
Glycine maxe1 - Gene
- nolY and nolZe0
- Outcome
- two open reading framese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“discovery of two open reading frames, designated nolY and nolZ”
e1“nodulation of soybeans, siratro, and cowpeas”
Claim · model-written summary
Inducible nolZ expression depended on NodD1 and NodW.
- Taxon
- soybeansGlycine maxe1
- Interactor
- Bradyrhizobium japonicum
Bradyrhizobium japonicumt0symbiont - Gene
- NodD1 and NodWe0
- Outcome
- inducible expressione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Studies utilizing a nolZ'-'lacZ fusion indicated that inducible expression is dependent upon both NodD1 and NodW”
e1“nodulation of soybeans, siratro, and cowpeas”
t0“Bradyrhizobium japonicum”
Claim · model-written summary
Deleting nolY produced slight nodulation defects in soybeans, siratro and cowpeas.
- Taxon
- soybeansGlycine maxe0
- Treatment
- A deletion mutation within nolYe0
- Interactor
- Bradyrhizobium japonicumBradyrhizobium japonicumt0symbiont
- Gene
- nolYe0
- Outcome
- defects in nodulatione0
- Direction
- defectse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A deletion mutation within nolY produced only slight defects in nodulation of soybeans, siratro, and cowpeas”
t0“Bradyrhizobium japonicum”
Claim · model-written summary
Deleting nolY produced stronger nodulation defects in mung beans.
- Taxon
- mung beans
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Vigna radiatae0 - Treatment
- A deletion mutation within nolYe1
- Interactor
- Bradyrhizobium japonicumBradyrhizobium japonicumt0symbiont
- Gene
- nolYe1
- Outcome
- defects were observed in nodulatione0
- Direction
- strongere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“stronger defects were observed in nodulation of mung beans”
e1“A deletion mutation within nolY produced only slight defects in nodulation of soybeans, siratro, and cowpeas”
t0“Bradyrhizobium japonicum”
Claim · model-written summary
A nolZ insertion caused no nodulation defects in tested hosts.
- Taxon
- soybeansGlycine maxe1
- Treatment
- An insertion mutation within nolZe0
- Interactor
- Bradyrhizobium japonicumBradyrhizobium japonicumt0symbiont
- Gene
- nolZe0
- Outcome
- nodulation defectse0
- Direction
- noe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An insertion mutation within nolZ showed no nodulation defects in the host plants tested”
e1“nodulation of soybeans, siratro, and cowpeas”
t0“Bradyrhizobium japonicum”
Claim · model-written summary
A nolY mutant showed no decrease in competitive nodule occupancy in soybeans.
- Taxon
- soybeansGlycine maxe0
- Interactor
- Bradyrhizobium japonicumBradyrhizobium japonicumt0symbiont
- Gene
- nolYe0
- Outcome
- competitiveness of a nolY mutante0
- Direction
- did not show any decreasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Competition assays for nodule occupancy in soybeans did not show any decrease in the competitiveness of a nolY mutant”
t0“Bradyrhizobium japonicum”
Claim · model-written summary
A nolY mutant had no detectable change in lipooligosaccharide nodulation signal production.
- Taxon
- soybeansGlycine maxe1
- Interactor
- Bradyrhizobium japonicumBradyrhizobium japonicumt0symbiont
- Gene
- nolYe0
- Outcome
- production of lipooligosaccharide nodulation signalse0
- Direction
- any detectable alteratione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nor did a nolY mutant show any detectable alteration in the production of lipooligosaccharide nodulation signals”
e1“nodule occupancy in soybeans”
t0“Bradyrhizobium japonicum”
The same questions in other papers
loss of function · Bradyrhizobium japonicum · Glycine max (soybean)Positive and negative results
9 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- negative
7 papers more in this group
See all 11 papers →loss of function · Bradyrhizobium japonicum · Vigna radiata (mung bean)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.