Abstract
Brazil is considered one of the domestication centers of cassava (Manihot esculenta), containing a large part of the biological diversity and traditional knowledge of the species. The aim of the present study was to evaluate the genetic diversity of cassava landraces grown by farmers in the north of Mato Grosso State, Brazil, using inter simple sequence repeat (ISSR) molecular markers. The study was carried out in the municipality of Alta Floresta, MT, on farms located in two rural areas. Seventeen cassava landraces were selected. The DNA was extracted and polymerase chain reaction amplifications were performed using 15 ISSR primers. Genetic similarity estimates were calculated using Jaccard's index and the generated matrix was used for clustering the genotypes by using UPGMA and Tocher's methods. The 15 ISSR primers amplified 120 fragments, revealing 61.67% polymorphism. The polymorphism information content ranged from 0.04 to 0.61, averaging 0.39. The most similar genotypes were AF5 and AF8, whereas the least similar were AF1 and AF16. The UPGMA clustering method formed five groups. Group I included twelve landraces, Group II contained two, and the other groups contained one landrace each. Tocher's method resulted in six groups: 12 landraces clustered in one group, and the other groups each contained one landrace. The ISSR markers proved efficient in revealing genetic diversity among the cassava landraces. The landraces grown by farmers in the two rural areas of Alta Floresta have a great variability and, thus, can be exploited in programs for breeding and preservation of the species.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 14
Claim · model-written summary
ISSR primers amplified 120 fragments with 61.67% polymorphism.
- Taxon
- cassava
Manihot esculentat0 - Outcome
- polymorphisme0
- Value
- 61.67%e0
- Qualifier
- 120 fragmentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 15 ISSR primers amplified 120 fragments, revealing 61.67% polymorphism”
t0“cassava”
Claim · model-written summary
Polymorphism information content ranged 0.04 to 0.61 and averaged 0.39.
- Taxon
- cassavaManihot esculentat0
- Outcome
- polymorphism information contente0
- Value
- 0.04 to 0.61e0
- Qualifier
- averaging 0.39e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The polymorphism information content ranged from 0.04 to 0.61, averaging 0.39”
t0“cassava”
Claim · model-written summary
AF5 and AF8 were most similar.
- Taxon
- cassavaManihot esculentat0
- Material
- AF5 and AF8e0
- Outcome
- most similar genotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The most similar genotypes were AF5 and AF8”
t0“cassava”
Claim · model-written summary
AF1 and AF16 were least similar.
- Taxon
- cassavaManihot esculentat0
- Material
- AF1 and AF16e0
- Outcome
- least similare0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the least similar were AF1 and AF16”
t0“cassava”
Claim · model-written summary
UPGMA formed five groups.
- Taxon
- cassavaManihot esculentat0
- Outcome
- five groupse0
- Value
- five groupse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The UPGMA clustering method formed five groups”
t0“cassava”
Claim · model-written summary
UPGMA Group I contained twelve landraces.
- Taxon
- cassavaManihot esculentat0
- Outcome
- Group Ie0
- Value
- twelve landracese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Group I included twelve landraces”
t0“cassava”
Claim · model-written summary
UPGMA Group II contained two landraces.
- Taxon
- cassavaManihot esculentat0
- Outcome
- Group IIe0
- Value
- twoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Group II contained two”
t0“cassava”
Claim · model-written summary
Other UPGMA groups contained one landrace each.
- Taxon
- cassavaManihot esculentat0
- Outcome
- other groupse0
- Value
- one landrace eache0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the other groups contained one landrace each”
t0“cassava”
Claim · model-written summary
Tocher clustering formed six groups.
- Taxon
- cassavaManihot esculentat0
- Outcome
- six groupse0
- Value
- six groupse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tocher's method resulted in six groups: 12 landraces clustered in one group, and the other groups each contained one landrace”
t0“cassava”
Claim · model-written summary
Tocher placed 12 landraces in one group.
- Taxon
- cassavaManihot esculentat0
- Outcome
- one groupe0
- Value
- 12 landracese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“12 landraces clustered in one group”
t0“cassava”
Claim · model-written summary
Other Tocher groups each contained one landrace.
- Taxon
- cassavaManihot esculentat0
- Outcome
- other groupse0
- Value
- one landracee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the other groups each contained one landrace”
t0“cassava”
Claim · model-written summary
ISSR markers revealed cassava landrace genetic diversity efficiently.
- Taxon
- cassavaManihot esculentae0
- Outcome
- genetic diversity among the cassava landracese0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ISSR markers proved efficient in revealing genetic diversity among the cassava landraces”
Claim · model-written summary
Farm landraces in two Alta Floresta rural areas showed great variability.
- Taxon
- cassavaManihot esculentat0
- Outcome
- great variabilitye0
- Setting
- Alta Florestae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The landraces grown by farmers in the two rural areas of Alta Floresta have a great variability”
t0“cassava”
Claim · model-written summary
Authors suggest using this variability for breeding and preservation.
- Taxon
- cassavaManihot esculentat0
- Outcome
- breeding and preservation of the speciese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“can be exploited in programs for breeding and preservation of the species”
t0“cassava”