Abstract
Cucurbita maxima is one of the most widely cultivated vegetables in China and exhibits distinct morphological characteristics. In this study, genetic linkage analysis with 57 simple-sequence repeats, 21 amplified fragment length polymorphisms, 3 random-amplified polymorphic DNA, and one morphological marker revealed 20 genetic linkage groups of C. maxima covering a genetic distance of 991.5 cM with an average of 12.1 cM between adjacent markers. Genetic linkage analysis identified the simple-sequence repeat marker 'PU078072' 5.9 cM away from the locus 'Rc', which controls rind color. The genetic map in the present study will be useful for better mapping, tagging, and cloning of quantitative trait loci/gene(s) affecting economically important traits and for breeding new varieties of C. maxima through marker-assisted selection.
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Claims 4
Claim · model-written summary
The linkage analysis revealed 20 genetic linkage groups of Cucurbita maxima.
- Taxon
- Cucurbita maxima
Cucurbita maximat0 - Outcome
- genetic linkage groupse0
- Value
- 20e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cucurbita maxima”
e0“revealed 20 genetic linkage groups of C. maxima”
Claim · model-written summary
The Cucurbita maxima linkage map covers a genetic distance of 991.5 cM.
- Taxon
- Cucurbita maximaCucurbita maximat0
- Outcome
- genetic distancee0
- Value
- 991.5 cMe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cucurbita maxima”
e0“covering a genetic distance of 991.5 cM”
Claim · model-written summary
The average distance between adjacent markers on the Cucurbita maxima linkage map is 12.1 cM.
- Taxon
- Cucurbita maximaCucurbita maximat0
- Outcome
- averagee0
- Value
- 12.1 cMe0
- Qualifier
- between adjacent markerse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cucurbita maxima”
e0“an average of 12.1 cM between adjacent markers”
Claim · model-written summary
The simple-sequence repeat marker 'PU078072' lies 5.9 cM from the locus 'Rc', which controls rind color in Cucurbita maxima.
- Taxon
- Cucurbita maximaCucurbita maximat0
- Gene
- 'Rc'e0
- Outcome
- simple-sequence repeat marker 'PU078072' 5.9 cM away from the locuse0
- Value
- 5.9 cMe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cucurbita maxima”
e0“Genetic linkage analysis identified the simple-sequence repeat marker 'PU078072' 5.9 cM away from the locus 'Rc', which controls rind color”