Abstract
Leaf photosynthesis in grapevines peaks approximately 30 to 40 days after unfolding and declines thereafter. In order to achieve high quality fruit is important that during the ripening stage the vine's photosynthetic capacity is not diminished due to the leaf senescence. To study grapevine leaf senescence at the molecular level, a transcription profiling analysis during leaf development and senescence was undertaken. A cDNA macroarray containing about 2300 putative unigenes was constructed and hybridized with RNA isolated from Vitis vinifera 'Pinot noir' leaves 7, 88 and 131 days-old. Data analysis focused on genes involved in the photosynthetic process or belonging to the proteinase family. At this stage of leaf senescence two gene categories were mainly affected: the chlorophyll a/b binding (CAB) genes and the cysteine-proteinases genes. While CAB transcripts decrease in their abundance, cysteine-proteinases are activated.
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Claims 3
Claim · model-written summary
CAB transcripts decreased in abundance during grapevine leaf senescence.
- Taxon
- Vitis vinifera
Vitis viniferae0 - Material
- 'Pinot noir'e0
- Organ / stage
- leaf senescencee3
- Gene
- chlorophyll a/b binding (CAB) genese2
- Outcome
- CAB transcriptse1
- Direction
- decreasee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Vitis vinifera 'Pinot noir' leaves 7, 88 and 131 days-old”
e1“While CAB transcripts decrease in their abundance, cysteine-proteinases are activated”
e2“chlorophyll a/b binding (CAB) genes and the cysteine-proteinases genes”
e3“At this stage of leaf senescence”
Claim · model-written summary
Cysteine-proteinase genes were activated during grapevine leaf senescence.
- Taxon
- Vitis vinifera
Vitis viniferae0 - Organ / stage
- leaf senescencee3
- Gene
- cysteine-proteinases genese2
- Outcome
- cysteine-proteinasese1
- Direction
- activatede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Vitis vinifera 'Pinot noir' leaves 7, 88 and 131 days-old”
e1“While CAB transcripts decrease in their abundance, cysteine-proteinases are activated”
e2“chlorophyll a/b binding (CAB) genes and the cysteine-proteinases genes”
e3“At this stage of leaf senescence”
Claim · model-written summary
A cDNA macroarray of about 2300 putative unigenes was constructed for grapevine leaves.
- Taxon
- Vitis vinifera
Vitis viniferae0 - Outcome
- cDNA macroarraye1
- Value
- about 2300 putative unigenese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Vitis vinifera 'Pinot noir' leaves 7, 88 and 131 days-old”
e1“A cDNA macroarray containing about 2300 putative unigenes was constructed”