Abstract
Early blight of potato caused by species of Alternaria is one of the most important diseases of potato worldwide. The occurrence of A. solani and A. alternata varies from region to region. The samples were collected from Himachal Pradesh, Uttar Pradesh and Madhya Pradesh states of India during 2009 and 2010. The identity of all the isolates was confirmed as A. alternata by morphological characters and sequencing. PCR amplification using universal primers ITS 4 and ITS 5 resulted in an amplicon of 594 bp which showed high similarity with reference ITS sequences of A. alternata in GenBank. Analysis of sequences revealed presence of partial sequence of ITS 1 and ITS 2 and complete sequence of 5.8S rRNA gene. All the isolates of A. alternata showed 100% similarity among themselves (except ALT 43) and with other A. alternata isolates in the GenBank. Three major clusters each were obtained when isolates were genotyped for RAPD and for AFLP markers. Pathogenicity was proved by detached leaf inoculation method and all the isolates were found pathogenic with varying degree of aggressiveness. Isolates were grouped into four groups on the basis of aggressiveness. No correlation was observed between aggressiveness and the clusters made on the basis of genotyping.
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Claims 9
Claim · model-written summary
All isolates were identified as A. alternata by morphology and sequencing.
- Taxon
- Potato
Solanum tuberosumt0 - Interactor
- A. alternata
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Alternaria alternatae0pathogen - Outcome
- identity of all the isolates was confirmed as A. alternatae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“The identity of all the isolates was confirmed as A. alternata by morphological characters and sequencing”
t0“Potato”
Claim · model-written summary
ITS amplification produced a 594 bp amplicon similar to GenBank reference sequences.
- Taxon
- PotatoSolanum tuberosumt0
- Interactor
- A. alternataAlternaria alternatae0pathogen
- Outcome
- amplicone0
- Value
- 594 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PCR amplification using universal primers ITS 4 and ITS 5 resulted in an amplicon of 594 bp which showed high similarity with reference ITS sequences of A. alternata in GenBank”
t0“Potato”
Claim · model-written summary
Sequences contained partial ITS 1 and ITS 2 and complete 5.8S rRNA gene sequences.
- Taxon
- PotatoSolanum tuberosumt0
- Interactor
- Alternaria alternataAlternaria alternatat1pathogen
- Outcome
- partial sequence of ITS 1 and ITS 2 and complete sequence of 5.8S rRNA genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“partial sequence of ITS 1 and ITS 2 and complete sequence of 5.8S rRNA gene”
t0“Potato”
t1“Alternaria alternata”
Claim · model-written summary
Isolates except ALT 43 showed 100% sequence similarity among themselves and GenBank isolates.
- Taxon
- PotatoSolanum tuberosumt0
- Interactor
- A. alternataAlternaria alternatae0pathogen
- Outcome
- similarity among themselvese0
- Value
- 100%e0
- Qualifier
- except ALT 43e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All the isolates of A. alternata showed 100% similarity among themselves (except ALT 43) and with other A. alternata isolates in the GenBank”
t0“Potato”
Claim · model-written summary
RAPD genotyping yielded three major isolate clusters.
- Taxon
- PotatoSolanum tuberosumt0
- Interactor
- Alternaria alternataAlternaria alternatat1pathogen
- Outcome
- clusterse0
- Value
- Threee0
- Qualifier
- RAPDe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three major clusters each were obtained when isolates were genotyped for RAPD and for AFLP markers”
t0“Potato”
t1“Alternaria alternata”
Claim · model-written summary
AFLP genotyping yielded three major isolate clusters.
- Taxon
- PotatoSolanum tuberosumt0
- Interactor
- Alternaria alternataAlternaria alternatat1pathogen
- Outcome
- clusterse0
- Value
- Threee0
- Qualifier
- AFLPe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three major clusters each were obtained when isolates were genotyped for RAPD and for AFLP markers”
t0“Potato”
t1“Alternaria alternata”
Claim · model-written summary
All isolates were pathogenic in detached potato leaves, with varying aggressiveness.
- Taxon
- PotatoSolanum tuberosumt0
- Interactor
- Alternaria alternataAlternaria alternatat1pathogen
- Outcome
- all the isolates were found pathogenic with varying degree of aggressivenesse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pathogenicity was proved by detached leaf inoculation method and all the isolates were found pathogenic with varying degree of aggressiveness”
t0“Potato”
t1“Alternaria alternata”
Claim · model-written summary
Isolates formed four aggressiveness groups.
- Taxon
- PotatoSolanum tuberosumt0
- Interactor
- Alternaria alternataAlternaria alternatat1pathogen
- Outcome
- groups on the basis of aggressivenesse0
- Value
- foure0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Isolates were grouped into four groups on the basis of aggressiveness”
t0“Potato”
t1“Alternaria alternata”
Claim · model-written summary
Aggressiveness did not correlate with genotype clusters.
- Taxon
- PotatoSolanum tuberosumt0
- Interactor
- Alternaria alternataAlternaria alternatat1pathogen
- Outcome
- aggressivenesse0
- Direction
- No correlatione0
- Comparator
- clusters made on the basis of genotypinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No correlation was observed between aggressiveness and the clusters made on the basis of genotyping”
t0“Potato”
t1“Alternaria alternata”