Paper · 2017 · Agronomy

Transferring a Biomass Enhancement Biotechnology from Glasshouse to Field: A Case Study on Wheat GWD RNAi

Alex Whan; Arunas Verbyla; Jos Mieog; et al.

3
claims
7
verbatim quotes
5
citations
2
research areas
✓
Open access · cc-by

Abstract

In glasshouse studies we have previously shown that endosperm-specific RNAi suppression of the primary starch phosphorylation enzyme, Glucan, Water Dikinase (GWD) leads to enhanced early vigor, greater leaf biomass, and increases in both head size and yield. To confirm these affects in a field setting, trials were conducted in three Australian environments. Field results were consistent with those in the glasshouse for increased flag leaf area and rachis nodes. However, there was also a decrease in tiller number and consequently a decrease in yield for one event at two sites. These findings provide potentially important information on plant vigor enhancement and highlight the challenges of transferring the modification of complex traits from single plants in controlled environments to the field.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 3

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

In field trials, GWD RNAi wheat showed increased flag leaf area and rachis nodes, consistent with glasshouse results.

Treatment
GWD RNAit0
Outcome
flag leaf area and rachis nodese1
Direction
increasede1
Setting
three Australian environmentse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Case Study on Wheat GWD RNAi”

✓ located verbatim in the source · title, characters 77–105 · show in text ↑
e0

“To confirm these affects in a field setting, trials were conducted in three Australian environments”

✓ located verbatim in the source · abstract, characters 259–358 · show in text ↑
e1

“Field results were consistent with those in the glasshouse for increased flag leaf area and rachis nodes”

✓ located verbatim in the source · abstract, characters 360–464 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

GWD RNAi wheat showed a decrease in tiller number at two sites for one event.

Treatment
GWD RNAit0
Outcome
tiller numbere2
Direction
decreasee2
Qualifier
for one event at two sitese2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Case Study on Wheat GWD RNAi”

✓ located verbatim in the source · title, characters 77–105 · show in text ↑
e2

“there was also a decrease in tiller number and consequently a decrease in yield for one event at two sites”

✓ located verbatim in the source · abstract, characters 475–581 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

GWD RNAi wheat showed a decrease in yield at two sites for one event.

Treatment
GWD RNAit0
Outcome
yielde2
Direction
decreasee2
Qualifier
for one event at two sitese2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Case Study on Wheat GWD RNAi”

✓ located verbatim in the source · title, characters 77–105 · show in text ↑
e2

“there was also a decrease in tiller number and consequently a decrease in yield for one event at two sites”

✓ located verbatim in the source · abstract, characters 475–581 · show in text ↑