Abstract
In glasshouse studies we have previously shown that endosperm-specific RNAi suppression of the primary starch phosphorylation enzyme, Glucan, Water Dikinase (GWD) leads to enhanced early vigor, greater leaf biomass, and increases in both head size and yield. To confirm these affects in a field setting, trials were conducted in three Australian environments. Field results were consistent with those in the glasshouse for increased flag leaf area and rachis nodes. However, there was also a decrease in tiller number and consequently a decrease in yield for one event at two sites. These findings provide potentially important information on plant vigor enhancement and highlight the challenges of transferring the modification of complex traits from single plants in controlled environments to the field.
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Claims 3
Claim · model-written summary
In field trials, GWD RNAi wheat showed increased flag leaf area and rachis nodes, consistent with glasshouse results.
- Taxon
- Wheat
Triticum aestivumt0 - Treatment
- GWD RNAit0
- Outcome
- flag leaf area and rachis nodese1
- Direction
- increasede1
- Setting
- three Australian environmentse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Case Study on Wheat GWD RNAi”
e0“To confirm these affects in a field setting, trials were conducted in three Australian environments”
e1“Field results were consistent with those in the glasshouse for increased flag leaf area and rachis nodes”
Claim · model-written summary
GWD RNAi wheat showed a decrease in tiller number at two sites for one event.
- Taxon
- Wheat
Triticum aestivumt0 - Treatment
- GWD RNAit0
- Outcome
- tiller numbere2
- Direction
- decreasee2
- Qualifier
- for one event at two sitese2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Case Study on Wheat GWD RNAi”
e2“there was also a decrease in tiller number and consequently a decrease in yield for one event at two sites”
Claim · model-written summary
GWD RNAi wheat showed a decrease in yield at two sites for one event.
- Taxon
- Wheat
Triticum aestivumt0 - Treatment
- GWD RNAit0
- Outcome
- yielde2
- Direction
- decreasee2
- Qualifier
- for one event at two sitese2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Case Study on Wheat GWD RNAi”
e2“there was also a decrease in tiller number and consequently a decrease in yield for one event at two sites”