Abstract
Ethylene acts as an inhibitor of the nodulation process of leguminous plants. However, some bacteria can decrease deleterious ethylene levels by the action of the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase which degrades ACC, the ethylene precursor in all higher plants. Co-inoculation of rhizobia with endophytes enhances the rhizobial symbiotic efficiency with legumes, improving both nodulation and nitrogen fixation. However, not much is understood about the mechanisms employed by these endophytic bacteria. In this regard, the role of ACC deaminase from endophytic strains in assisting rhizobia in this process has yet to be confirmed. In this study, the role of ACC deaminase in an endophyte's ability to increase Rhizobium tropici nodulation of common bean was evaluated. To assess the effect of ACC deaminase in an endophyte's ability to promote rhizobial nodulation, the endophyte Serratia grimesii BXF1, which does not encode ACC deaminase, was transformed with an exogenous acdS gene. The results obtained indicate that the ACC deaminase-overexpressing transformant strain increased common bean growth, and enhanced the nodulation abilities of R. tropici CIAT899, in both cases compared to the wild-type non-transformed strain. Furthermore, plant inoculation with the ACC deaminase-overproducing strain led to an increased level of plant protection against a seed-borne pathogen. SIGNIFICANCE AND IMPACT OF THE STUDY: In this work, we studied the effect of ACC deaminase production by the bacterial endophyte Serratia grimesi BXF1, and its impact on the nodulation process of common bean. The results obtained indicate that ACC deaminase is an asset to the synergetic interaction between rhizobia and the endophyte, positively contributing to the overall legume-rhizobia symbiosis by regulating inhibitory ethylene levels that might otherwise inhibit nodulation and overall plant growth. The use of rhizobia together with an ACC deaminase-producing endophyte is, therefore, an important strategy for the development of new bacterial inoculants with increased performance.
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Claims 4
Claim · model-written summary
The ACC deaminase overexpressing endophyte increased common bean growth relative to its wild type.
- Taxon
- common bean
Phaseolus vulgarise0 - Treatment
- ACC deaminase-overexpressing transformant straine0
- Interactor
- Serratia grimesii BXF1Serratia grimesiie2symbiont
- Outcome
- common bean growthe0
- Direction
- increasede0
- Comparator
- wild-type non-transformed straine1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ACC deaminase-overexpressing transformant strain increased common bean growth”
e1“compared to the wild-type non-transformed strain”
e2“endophyte Serratia grimesii BXF1”
Claim · model-written summary
The transformed endophyte improved R. tropici CIAT899 nodulation compared with its wild type.
- Taxon
- common beanPhaseolus vulgarise3
- Treatment
- ACC deaminase-overexpressing transformant straine1
- Interactor
- R. tropici CIAT899
Martinezella tropicie0symbiont - Second interactor
- Serratia grimesii BXF1Serratia grimesiie4symbiont
- Outcome
- nodulation abilities of R. tropici CIAT899e0
- Direction
- enhancede0
- Comparator
- wild-type non-transformed straine2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enhanced the nodulation abilities of R. tropici CIAT899”
e1“ACC deaminase-overexpressing transformant strain”
e2“compared to the wild-type non-transformed strain”
e3“nodulation of common bean was evaluated”
e4“Serratia grimesii BXF1”
Claim · model-written summary
Inoculation with the ACC deaminase producing strain increased protection against a seed borne pathogen.
- Taxon
- common beanPhaseolus vulgarise1
- Treatment
- plant inoculation with the ACC deaminase-overproducing straine0
- Interactor
- seed-borne pathogene0pathogen
- Outcome
- plant protection against a seed-borne pathogene0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plant inoculation with the ACC deaminase-overproducing strain led to an increased level of plant protection against a seed-borne pathogen”
e1“nodulation of common bean was evaluated”
Claim · model-written summary
The authors propose combining rhizobia and ACC deaminase producing endophytes as improved inoculants.
- Taxon
- common beanPhaseolus vulgarise1
- Interactor
- rhizobiae0symbiont
- Outcome
- new bacterial inoculants with increased performancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The use of rhizobia together with an ACC deaminase-producing endophyte is, therefore, an important strategy for the development of new bacterial inoculants with increased performance.”
e1“nodulation of common bean was evaluated”
The same questions in other papers
inoculation · control · Phaseolus vulgaris (common bean)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.