Paper · 2017 · Journal of Microbial & Biochemical Technology

Comparison of IAA Production by Shallot Rhizosphere Isolated Bacteria in Solid and Liquid Media and Their Effect on Shallot Plant Growth

Kafrawi; Nildayanti; Zahraeni K; et al.

14
claims
28
verbatim quotes
12
citations
2
research areas
✓
Open access

Abstract

Some of free-living rhizobacteria were isolated from rhizosphereof shallot growing in two different fields on Sulawesi Island. The bacterial isolates were cultured in liquid and solid media and were further tested for their ability to produce bioauxin. L-Tryptophan as a physiological precursor of auxin was added to the culture media and the production of IAA was tested using a colorimeter method. Six isolates from west Sulawesi and ten isolates from South Sulawesi were found to produce bioauxin, while 40 other isolates had negative results on IAA production. The amount of IAA produced by the isolates on liquid medium ranged from 4.01 to 8.62 ppm, while in the solid medium, the concentration of IAA produced by the same bacteria was considerably lower than in Liquid media. Thus, culture conditions influence the secretion of IAA by the bacteria. Of these 16 IAA producing isolates, five efficient producers were used for in plant a growth promotion assay. The isolate MK6-1-1 showed the best stimulatory effect on leaves number and bulb tillers, isolate LB8 was investigated to modulate better bulbs fresh biomass weight, while isolate MK11 was found to have the best effect on bulbs dry weight and bulbs dry biomass weight. Application of liquid media MK 6-1-1 bacteria isolates on the medium shallot planting early phases provides the best results on the vegetative growth of shallot that is leafs number (10.75 pieces) and the number of bulb tillers (2.75 bulbs) except the best plants height showed by Isolate bacteria MK 11 (29,60 cm). Bacterial isolates LB 8 shows bulbs fresh biomass weight (9.83 g) and bulbs fresh biomass shrinkage (7.45) while the bacteria Isolate MK 11 showed the best result of bulb dry weight (2.26 g) and bulbs dry biomass weight (3.41).

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Claims 14

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Six western Sulawesi and ten South Sulawesi rhizobacterial isolates produced bioauxin.

Interactor
isolatese0symbiont
Outcome
produce bioauxine0
Setting
west Sulawesie0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa var. aggregatum
Allium cepa var. aggregatum · TaxonEvan-Amos · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Six isolates from west Sulawesi and ten isolates from South Sulawesi were found to produce bioauxin, while 40 other isolates had negative results on IAA production.”

✓ located verbatim in the source · abstract, characters 400–564 · show in text ↑
t0

“Shallot Rhizosphere”

✓ located verbatim in the source · title, characters 32–51 · show in text ↑
Observationobservedoriginal resultno effectmeasured

Claim · model-written summary

Forty other isolates did not produce IAA.

Interactor
isolatese0symbiont
Outcome
negative results on IAA productione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa var. aggregatum
Allium cepa var. aggregatum · TaxonEvan-Amos · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Six isolates from west Sulawesi and ten isolates from South Sulawesi were found to produce bioauxin, while 40 other isolates had negative results on IAA production.”

✓ located verbatim in the source · abstract, characters 400–564 · show in text ↑
t0

“Shallot Rhizosphere”

✓ located verbatim in the source · title, characters 32–51 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Isolates produced 4.01 to 8.62 ppm IAA in liquid medium.

Interactor
isolatese0symbiont
Outcome
IAA produced by the isolatese0
Value
4.01 to 8.62 ppme0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa var. aggregatum
Allium cepa var. aggregatum · TaxonEvan-Amos · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The amount of IAA produced by the isolates on liquid medium ranged from 4.01 to 8.62 ppm, while in the solid medium, the concentration of IAA produced by the same bacteria was considerably lower than in Liquid media.”

✓ located verbatim in the source · abstract, characters 565–781 · show in text ↑
t0

“Shallot Rhizosphere”

✓ located verbatim in the source · title, characters 32–51 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

IAA production was lower in solid than liquid medium.

Treatment
solid mediume0
Interactor
bacteriaBacteriae0symbiont
Outcome
concentration of IAA produced by the same bacteriae0
Direction
lowere0
Comparator
Liquid mediae0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa var. aggregatum
Allium cepa var. aggregatum · TaxonEvan-Amos · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The amount of IAA produced by the isolates on liquid medium ranged from 4.01 to 8.62 ppm, while in the solid medium, the concentration of IAA produced by the same bacteria was considerably lower than in Liquid media.”

✓ located verbatim in the source · abstract, characters 565–781 · show in text ↑
t0

“Shallot Rhizosphere”

✓ located verbatim in the source · title, characters 32–51 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Shallot growth response reported for leaves number and bulb tillers.

Treatment
MK6-1-1e0
Interactor
MK6-1-1e0symbiont
Outcome
leaves number and bulb tillerse0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa var. aggregatum
Allium cepa var. aggregatum · TaxonEvan-Amos · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The isolate MK6-1-1 showed the best stimulatory effect on leaves number and bulb tillers”

✓ located verbatim in the source · abstract, characters 965–1053 · show in text ↑
t0

“Shallot Rhizosphere”

✓ located verbatim in the source · title, characters 32–51 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Shallot growth response reported for bulbs fresh biomass weight.

Treatment
LB8e0
Interactor
LB8e0symbiont
Outcome
bulbs fresh biomass weighte0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa var. aggregatum
Allium cepa var. aggregatum · TaxonEvan-Amos · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“isolate LB8 was investigated to modulate better bulbs fresh biomass weight”

✓ located verbatim in the source · abstract, characters 1055–1129 · show in text ↑
t0

“Shallot Rhizosphere”

✓ located verbatim in the source · title, characters 32–51 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Shallot growth response reported for bulbs dry weight and bulbs dry biomass weight.

Treatment
MK11e0
Interactor
MK11e0symbiont
Outcome
bulbs dry weight and bulbs dry biomass weighte0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa var. aggregatum
Allium cepa var. aggregatum · TaxonEvan-Amos · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“isolate MK11 was found to have the best effect on bulbs dry weight and bulbs dry biomass weight”

✓ located verbatim in the source · abstract, characters 1137–1232 · show in text ↑
t0

“Shallot Rhizosphere”

✓ located verbatim in the source · title, characters 32–51 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Shallot growth response reported for leafs number.

Outcome
leafs numbere0
Value
10.75 piecese0

Not reportedInteractorInteractor roleTreatmentComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa var. aggregatum
Allium cepa var. aggregatum · TaxonEvan-Amos · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“leafs number (10.75 pieces)”

✓ located verbatim in the source · abstract, characters 1403–1430 · show in text ↑
t0

“Shallot Rhizosphere”

✓ located verbatim in the source · title, characters 32–51 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Shallot growth response reported for number of bulb tillers.

Outcome
number of bulb tillerse0
Value
2.75 bulbse0

Not reportedInteractorInteractor roleTreatmentComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa var. aggregatum
Allium cepa var. aggregatum · TaxonEvan-Amos · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the number of bulb tillers (2.75 bulbs)”

✓ located verbatim in the source · abstract, characters 1435–1474 · show in text ↑
t0

“Shallot Rhizosphere”

✓ located verbatim in the source · title, characters 32–51 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Shallot growth response reported for plants height.

Treatment
MK 11e0
Interactor
MK 11e0symbiont
Outcome
plants heighte0
Value
29,60 cme0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa var. aggregatum
Allium cepa var. aggregatum · TaxonEvan-Amos · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“plants height showed by Isolate bacteria MK 11 (29,60 cm)”

✓ located verbatim in the source · abstract, characters 1491–1548 · show in text ↑
t0

“Shallot Rhizosphere”

✓ located verbatim in the source · title, characters 32–51 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Shallot growth response reported for bulbs fresh biomass weight.

Treatment
LB 8e0
Interactor
LB 8e0symbiont
Outcome
bulbs fresh biomass weighte0
Value
9.83 ge0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa var. aggregatum
Allium cepa var. aggregatum · TaxonEvan-Amos · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Bacterial isolates LB 8 shows bulbs fresh biomass weight (9.83 g)”

✓ located verbatim in the source · abstract, characters 1550–1615 · show in text ↑
t0

“Shallot Rhizosphere”

✓ located verbatim in the source · title, characters 32–51 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Shallot growth response reported for bulbs fresh biomass shrinkage.

Outcome
bulbs fresh biomass shrinkagee0
Value
7.45e0

Not reportedInteractorInteractor roleTreatmentComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa var. aggregatum
Allium cepa var. aggregatum · TaxonEvan-Amos · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“bulbs fresh biomass shrinkage (7.45)”

✓ located verbatim in the source · abstract, characters 1620–1656 · show in text ↑
t0

“Shallot Rhizosphere”

✓ located verbatim in the source · title, characters 32–51 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Shallot growth response reported for bulb dry weight.

Treatment
MK 11e0
Interactor
MK 11e0symbiont
Outcome
bulb dry weighte0
Value
2.26 ge0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa var. aggregatum
Allium cepa var. aggregatum · TaxonEvan-Amos · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“bacteria Isolate MK 11 showed the best result of bulb dry weight (2.26 g)”

✓ located verbatim in the source · abstract, characters 1667–1740 · show in text ↑
t0

“Shallot Rhizosphere”

✓ located verbatim in the source · title, characters 32–51 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Shallot growth response reported for bulbs dry biomass weight.

Outcome
bulbs dry biomass weighte0
Value
3.41e0

Not reportedInteractorInteractor roleTreatmentComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa var. aggregatum
Allium cepa var. aggregatum · TaxonEvan-Amos · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“bulbs dry biomass weight (3.41)”

✓ located verbatim in the source · abstract, characters 1745–1776 · show in text ↑
t0

“Shallot Rhizosphere”

✓ located verbatim in the source · title, characters 32–51 · show in text ↑

in vitro · Bacteria · Allium cepa (onion)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. negative
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.