Abstract
The world potato is facing major economic losses due to disease pressure and environmental concerns regarding pesticides use. This work aims at addressing these two issues by isolating indigenous bacteria that can be integrated into pest management strategies. More than 2,800 strains of Bacillus-like and Pseudomonas-like were isolated from several soils and substrates associated with potato agro-systems in Belgium. Screenings for antagonistic activities against the potato pathogens Alternaria solani, Fusarium solani (BCCM-MUCL5492), Pectobacterium carotovorum (ATCC 15713), Phytophthora infestans (CRA-W10022) and Rhizoctonia solani (BCCM-MUCL 51929) were performed, allowing the selection of 52 Bacillus spp. and eight Pseudomonas spp. displaying growth inhibition of at least 50% under in vitro conditions, particularly against P. infestans. All 60 bacterial isolates were identified based on 16S rRNA gene sequencing and further characterized for the production of potential bio-active secondary metabolites. The antagonistic activities displayed by the selected strains indicated that versatile metabolites can be produced by the strains. For instance, the detection of genes involved bacilysin biosynthesis was correlated with the strong antagonism of Bacillus pumilus strains towards P. infestans, whereas the production of both bio-surfactants and siderophores might explain the high antagonistic activities against late blight. Greenhouse assays with potato plants were performed with the most effective strains (seven Bacillus spp. and four Pseudomonas spp.) in order to evaluate their in vivo antagonistic effect against P. infestans. Based on these results, four strains (Bacillus amyloliquefaciens 17A-B3, Bacillus subtilis 30B-B6, Pseudomonas brenneri 43R-P1 and Pseudomonas protegens 44R-P8) were retained for further evaluation of their protection index against P. infestans in a pilot field trial. Interestingly, B. subtilis 30B-B6 was shown to significantly decrease late blight severity throughout the crop season. Overall, this study showed that antagonistic indigenous soil bacteria can offer an alternative to the indiscriminate use of pesticide in potato agro-systems.
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Claims 10
Claim · model-written summary
More than 2,800 Bacillus-like and Pseudomonas-like strains were isolated from potato-associated soils and substrates in Belgium.
Verbatim
e0“More than 2,800 strains of Bacillus-like and Pseudomonas-like were isolated from several soils and substrates associated with potato agro-systems in Belgium”
Claim · model-written summary
Screening selected 52 Bacillus isolates with at least 50% pathogen growth inhibition in vitro, particularly against P. infestans.
- Taxon
- Bacillus spp.e0
- Interactor
- P. infestans
Phytophthora infestanse0pathogen - Outcome
- growth inhibitione0
- Value
- at least 50%e0
- Qualifier
- 52e0
- Setting
- in vitro conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“allowing the selection of 52 Bacillus spp. and eight Pseudomonas spp. displaying growth inhibition of at least 50% under in vitro conditions, particularly against P. infestans”
Claim · model-written summary
Screening selected eight Pseudomonas isolates with at least 50% pathogen growth inhibition in vitro, particularly against P. infestans.
- Taxon
- Pseudomonas spp.
Pseudomonase0 - Interactor
- P. infestansPhytophthora infestanse0pathogen
- Outcome
- growth inhibitione0
- Value
- at least 50%e0
- Qualifier
- eighte0
- Setting
- in vitro conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“allowing the selection of 52 Bacillus spp. and eight Pseudomonas spp. displaying growth inhibition of at least 50% under in vitro conditions, particularly against P. infestans”
Claim · model-written summary
All 60 bacterial isolates were identified using 16S rRNA sequencing.
Verbatim
e0“All 60 bacterial isolates were identified based on 16S rRNA gene sequencing”
Claim · model-written summary
Selected bacterial strains showed antagonistic activities suggesting production of versatile metabolites.
Verbatim
e0“The antagonistic activities displayed by the selected strains indicated that versatile metabolites can be produced by the strains”
e1“All 60 bacterial isolates”
Claim · model-written summary
Detection of bacilysin biosynthesis genes correlated with strong Bacillus pumilus antagonism toward P. infestans.
- Taxon
- Bacillus pumilusBacillus pumiluse0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Gene
- genes involved bacilysin biosynthesise0
- Outcome
- strong antagonisme0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the detection of genes involved bacilysin biosynthesis was correlated with the strong antagonism of Bacillus pumilus strains towards P. infestans”
Claim · model-written summary
Bio-surfactant and siderophore production might explain high antagonistic activity against late blight.
Verbatim
e0“the production of both bio-surfactants and siderophores might explain the high antagonistic activities against late blight”
e1“All 60 bacterial isolates”
Claim · model-written summary
Four bacterial strains were retained for field protection evaluation against P. infestans after greenhouse assays.
- Taxon
- P. infestansPhytophthora infestanse0
- Outcome
- retained for further evaluatione0
- Value
- four strainse0
- Qualifier
- Bacillus amyloliquefaciens 17A-B3, Bacillus subtilis 30B-B6, Pseudomonas brenneri 43R-P1 and Pseudomonas protegens 44R-P8e0
- Setting
- pilot field triale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“four strains (Bacillus amyloliquefaciens 17A-B3, Bacillus subtilis 30B-B6, Pseudomonas brenneri 43R-P1 and Pseudomonas protegens 44R-P8) were retained for further evaluation of their protection index against P. infestans in a pilot field trial”
Claim · model-written summary
B. subtilis 30B-B6 significantly decreased potato late blight severity throughout the crop season.
- Taxon
- Potato
Solanum tuberosumt0 - Treatment
- B. subtilis 30B-B6e0
- Interactor
- P. infestansPhytophthora infestanse1pathogen
- Second interactor
- B. subtilis 30B-B6
Bacillus subtilise0biocontrol_agent - Outcome
- late blight severitye0
- Direction
- decreasee0
- Qualifier
- throughout the crop seasone0
- Setting
- pilot field triale1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“B. subtilis 30B-B6 was shown to significantly decrease late blight severity throughout the crop season”
e1“against P. infestans in a pilot field trial”
t0“Potato”
Claim · model-written summary
The authors conclude that indigenous antagonistic soil bacteria offer an alternative to indiscriminate pesticide use in potato production.
- Taxon
- potatoSolanum tuberosume0
- Interactor
- antagonistic indigenous soil bacteriae0biocontrol_agent
- Outcome
- alternative to the indiscriminate use of pesticidee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“antagonistic indigenous soil bacteria can offer an alternative to the indiscriminate use of pesticide in potato agro-systems”
The same questions in other papers
Bacillus subtilis · Phytophthora infestans · Solanum tuberosum (potato)Results agree
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See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.