Abstract
Nontranscribed spacers (NTS) of 5S rDNA are often polymorphic in closely related species and even in the same genome. The polymorphism of 5S rDNA NTS was shown between genomes St, J, and V of Triticeae species Thinopyrum bessarabicum, Pseudoroegneria spicata, and Dasypyrum villosum, respectively. A molecular genetic marker was designed based on the 5S rDNA NTS polymorphism that allows identification of the St, J, and V genomes. We designed a pair of primers that correspond to the conserved regions of 5S rDNA NTS between the genomes studied. The PCR amplicon length is 158 bp, 171 bp, and 172 bp for V, St, and J genomes, respectively. The fragment of the St genome is characterized by the SmiM I restriction site that enables its differentiation from the J genome fragment that lacks this site. The developed marker showed its efficiency for verification of germplasm accessions and the study of allopolyploids.
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Claims 7
Claim · model-written summary
5S rDNA NTS was polymorphic among St, J and V genomes.
- Taxon
- Thinopyrum bessarabicumThinopyrum bessarabicume0
- Gene
- 5S rDNA NTSe0
- Outcome
- polymorphisme0
Verbatim
e0“The polymorphism of 5S rDNA NTS was shown between genomes St, J, and V of Triticeae species Thinopyrum bessarabicum, Pseudoroegneria spicata, and Dasypyrum villosum”
Claim · model-written summary
The marker identified St, J and V genomes.
- Taxon
- Thinopyrum bessarabicumThinopyrum bessarabicumt0
- Gene
- 5S rDNA NTSe0
- Outcome
- identification of the St, J, and V genomese0
Not reportedComparatorValue
Verbatim
e0“A molecular genetic marker was designed based on the 5S rDNA NTS polymorphism that allows identification of the St, J, and V genomes”
t0“Thinopyrum bessarabicum”
Claim · model-written summary
The V genome amplicon was 158 bp.
- Taxon
- Dasypyrum villosum
Dasypyrum villosumt0 - Material
- Ve0
- Outcome
- PCR amplicon lengthe0
- Value
- 158 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The PCR amplicon length is 158 bp, 171 bp, and 172 bp for V, St, and J genomes, respectively”
t0“Dasypyrum villosum”
Claim · model-written summary
The St genome amplicon was 171 bp.
- Taxon
- Pseudoroegneria spicata
.jpg?width=60)
Pseudoroegneria spicatat0 - Material
- Ste0
- Outcome
- PCR amplicon lengthe0
- Value
- 171 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“The PCR amplicon length is 158 bp, 171 bp, and 172 bp for V, St, and J genomes, respectively”
t0“Pseudoroegneria spicata”
Claim · model-written summary
The J genome amplicon was 172 bp.
- Taxon
- Thinopyrum bessarabicumThinopyrum bessarabicumt0
- Material
- Je0
- Outcome
- PCR amplicon lengthe0
- Value
- 172 bpe0
Verbatim
e0“The PCR amplicon length is 158 bp, 171 bp, and 172 bp for V, St, and J genomes, respectively”
t0“Thinopyrum bessarabicum”
Claim · model-written summary
The St fragment had an SmiM I site absent in the J fragment.
- Taxon
- Pseudoroegneria spicataPseudoroegneria spicatat0
- Material
- Ste0
- Gene
- SmiM Ie0
- Outcome
- restriction sitee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The fragment of the St genome is characterized by the SmiM I restriction site that enables its differentiation from the J genome fragment that lacks this site”
t0“Pseudoroegneria spicata”
Claim · model-written summary
The marker was effective for verifying germplasm accessions and studying allopolyploids.
- Taxon
- Thinopyrum bessarabicumThinopyrum bessarabicumt0
- Outcome
- efficiency for verification of germplasm accessions and the study of allopolyploidse0
Not reportedComparatorValue
Verbatim
e0“The developed marker showed its efficiency for verification of germplasm accessions and the study of allopolyploids”
t0“Thinopyrum bessarabicum”