Abstract
Ubiquitination plays key roles in eukaryotic growth, stress adaptation, and metabolic regulation. In our previous work, ubiquitin was found to be secreted in the hypovirus-infected strain of Cryphonectria parasitica, a phytopathogenic filamentous fungus responsible for the chestnut blight. Here we report the functional and molecular characterization of a polyubiquitin gene, cpubi4, in C. parasitica. The expression of cpubi4 was upregulated by the infection of a hypovirus. Deletion of cpubi4 resulted in abnormal morphology, reduced sporulation, attenuation of virulence, and significant reduction in ubiquitination. A total of 378 sites in 236 proteins were identified to be significantly decreased in ubiquitination in the absence of cpubi4. Quantitative proteome analysis revealed that 285 in 4776 identified proteins changed in abundance (1.5-fold, P<0.05) in the cpubi4 null mutant, as compared with the wild-type strain.
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Claims 7
Claim · model-written summary
Expression of cpubi4 in Cryphonectria parasitica is upregulated by hypovirus infection.
- Taxon
- Chestnutt0
- Treatment
- the infection of a hypoviruse1
- Gene
- cpubi4e1
- Outcome
- expression of cpubi4e1
- Direction
- upregulatede1
Not reportedComparator
Verbatim
t0“Chestnut Blight Fungus”
e1“The expression of cpubi4 was upregulated by the infection of a hypovirus”
Claim · model-written summary
Deletion of cpubi4 gives abnormal morphology in Cryphonectria parasitica.
- Taxon
- Chestnutt0
- Treatment
- Deletion of cpubi4e2
- Interactor
- Cryphonectria parasitica
Cryphonectria parasiticae0pathogen - Gene
- cpubi4e2
- Outcome
- abnormal morphologye2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Chestnut Blight Fungus”
e0“hypovirus-infected strain of Cryphonectria parasitica”
e2“Deletion of cpubi4 resulted in abnormal morphology, reduced sporulation, attenuation of virulence, and significant reduction in ubiquitination”
Claim · model-written summary
Deletion of cpubi4 reduces sporulation of Cryphonectria parasitica.
- Taxon
- Chestnutt0
- Treatment
- Deletion of cpubi4e2
- Interactor
- Cryphonectria parasiticaCryphonectria parasiticae0pathogen
- Gene
- cpubi4e2
- Outcome
- sporulatione2
- Direction
- reducede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Chestnut Blight Fungus”
e0“hypovirus-infected strain of Cryphonectria parasitica”
e2“Deletion of cpubi4 resulted in abnormal morphology, reduced sporulation, attenuation of virulence, and significant reduction in ubiquitination”
Claim · model-written summary
Deletion of cpubi4 attenuates the virulence of Cryphonectria parasitica on chestnut.
- Taxon
- Chestnutt0
- Treatment
- Deletion of cpubi4e2
- Interactor
- Cryphonectria parasiticaCryphonectria parasiticae0pathogen
- Gene
- cpubi4e2
- Outcome
- virulencee2
- Direction
- attenuatione2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Chestnut Blight Fungus”
e0“hypovirus-infected strain of Cryphonectria parasitica”
e2“Deletion of cpubi4 resulted in abnormal morphology, reduced sporulation, attenuation of virulence, and significant reduction in ubiquitination”
Claim · model-written summary
Deletion of cpubi4 significantly reduces ubiquitination in Cryphonectria parasitica.
- Taxon
- Chestnutt0
- Treatment
- Deletion of cpubi4e2
- Interactor
- Cryphonectria parasiticaCryphonectria parasiticae0pathogen
- Gene
- cpubi4e2
- Outcome
- ubiquitinatione2
- Direction
- reductione2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Chestnut Blight Fungus”
e0“hypovirus-infected strain of Cryphonectria parasitica”
e2“Deletion of cpubi4 resulted in abnormal morphology, reduced sporulation, attenuation of virulence, and significant reduction in ubiquitination”
Claim · model-written summary
In the absence of cpubi4, 378 sites in 236 proteins are significantly decreased in ubiquitination.
- Taxon
- Chestnutt0
- Treatment
- absence of cpubi4e3
- Interactor
- Cryphonectria parasiticaCryphonectria parasiticae0pathogen
- Gene
- cpubi4e3
- Outcome
- ubiquitinatione3
- Direction
- decreasede3
- Value
- 378 sitese3
- Qualifier
- in 236 proteinse3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Chestnut Blight Fungus”
e0“hypovirus-infected strain of Cryphonectria parasitica”
e3“A total of 378 sites in 236 proteins were identified to be significantly decreased in ubiquitination in the absence of cpubi4”
Claim · model-written summary
In the cpubi4 null mutant, 285 of 4776 identified proteins changed in abundance compared with the wild-type strain.
- Taxon
- Chestnutt0
- Treatment
- cpubi4 null mutante4
- Interactor
- Cryphonectria parasiticaCryphonectria parasiticae0pathogen
- Outcome
- abundancee4
- Direction
- changede4
- Value
- 285e4
- Qualifier
- 1.5-fold, P<0.05e4
- Comparator
- wild-type straine4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Chestnut Blight Fungus”
e0“hypovirus-infected strain of Cryphonectria parasitica”
e4“Quantitative proteome analysis revealed that 285 in 4776 identified proteins changed in abundance (1.5-fold, P<0.05) in the cpubi4 null mutant, as compared with the wild-type strain”
The same questions in other papers
loss of function · Cryphonectria parasitica · CastaneaResults agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.