Paper · 2018 · Grassland Science

Influence of radiation on Hemarthria compressa's genetic variations

Bingchao Wu; Yu Zhang; Haidong Yan; et al.

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claims
11
verbatim quotes
0
citations
2
research areas
—
Not open access

Abstract

Abstract Using the material of Hemarthria compressa (L.F.) R.Br. cv. YA ’ AN , we carried out this research to study the influence of radiation on the genetic variation of plants. Genetic difference was analyzed with expressed sequence tag‐simple sequence repeat (EST‐SSR) molecular marker through the comparison of 60 Co‐ γ radiation on H. compressa seed stems and original variety. By using 20 primer pairs, 176 polymerase chain reaction (PCR)‐amplifications with clear and consistent bands were obtained. The results showed that 155 of 176 bands were polymorphic, which indicating an 88.07% polymorphism rate, and each pair of primers had 8.8 amplified bands on average; the amplitude of polymorphism information content was 0.4709–0.6952 with an average value 0.6081. The genetic similarity coefficient of H. compressa and its mutants ranged from 0.4318 to 0.8239 with an average of 0.6671. As a consequence, existence of genetic differences between the mutants and the basic material was proved.

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Claims 6

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Twenty primer pairs gave 176 clear consistent PCR amplification bands.

Taxon
Hemarthria compressaHemarthria compressat0
Outcome
polymerase chain reaction (PCR)‐amplifications with clear and consistent bandse0
Value
176e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hemarthria compressa
Hemarthria compressa · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

e0

“By using 20 primer pairs, 176 polymerase chain reaction (PCR)‐amplifications with clear and consistent bands were obtained.”

✓ located verbatim in the source · abstract, characters 384–507 · show in text ↑
t0

“Hemarthria compressa”

✓ located verbatim in the source · title, characters 26–46 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Of 176 bands, 155 were polymorphic, a rate of 88.07%.

Taxon
Hemarthria compressaHemarthria compressat0
Outcome
polymorphism ratee0
Value
88.07%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hemarthria compressa
Hemarthria compressa · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

e0

“155 of 176 bands were polymorphic, which indicating an 88.07% polymorphism rate”

✓ located verbatim in the source · abstract, characters 532–611 · show in text ↑
t0

“Hemarthria compressa”

✓ located verbatim in the source · title, characters 26–46 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Each primer pair averaged 8.8 amplified bands.

Taxon
Hemarthria compressaHemarthria compressat0
Outcome
amplified bands on averagee0
Value
8.8e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hemarthria compressa
Hemarthria compressa · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

e0

“each pair of primers had 8.8 amplified bands on average”

✓ located verbatim in the source · abstract, characters 617–672 · show in text ↑
t0

“Hemarthria compressa”

✓ located verbatim in the source · title, characters 26–46 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Polymorphism information content ranged from 0.4709 to 0.6952, averaging 0.6081.

Taxon
Hemarthria compressaHemarthria compressat0
Outcome
polymorphism information contente0
Value
0.4709–0.6952e0
Qualifier
average value 0.6081e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hemarthria compressa
Hemarthria compressa · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

e0

“the amplitude of polymorphism information content was 0.4709–0.6952 with an average value 0.6081”

✓ located verbatim in the source · abstract, characters 674–770 · show in text ↑
t0

“Hemarthria compressa”

✓ located verbatim in the source · title, characters 26–46 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Mutant and original H. compressa similarity coefficients ranged from 0.4318 to 0.8239, averaging 0.6671.

Taxon
H. compressaHemarthria compressae0
Outcome
genetic similarity coefficiente0
Value
0.4318 to 0.8239e0
Qualifier
average of 0.6671e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hemarthria compressa
Hemarthria compressa · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

e0

“The genetic similarity coefficient of H. compressa and its mutants ranged from 0.4318 to 0.8239 with an average of 0.6671.”

✓ located verbatim in the source · abstract, characters 772–894 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Mutants differed genetically from the basic material.

Taxon
Hemarthria compressaHemarthria compressat0
Outcome
genetic differences between the mutants and the basic materiale0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hemarthria compressa
Hemarthria compressa · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

e0

“existence of genetic differences between the mutants and the basic material was proved”

✓ located verbatim in the source · abstract, characters 913–999 · show in text ↑
t0

“Hemarthria compressa”

✓ located verbatim in the source · title, characters 26–46 · show in text ↑