Abstract
In 2011–2014, ELISA or nucleic acid spot hybridization (NASH) testing for common potato viruses or Potato spindle tuber viroid (PSTVd) was performed on 500 leaf samples collected in potato fields in the northeast provinces Heilongjiang and Inner Mongolia, China. The results revealed that 38.4% (Heilongjiang) and 27.7% (Inner Mongolia) were positive for Potato virus Y (PVY). To unveil the strain composition and population structure of PVY in the region, the multiplex RT-PCR described by Chikh-Ali et al. was performed on all of the ELISA-PVY-positive samples. Of the 158 samples whose PVY strain scenarios could be determined, PVY NTN-NW -SYR-II and PVY N-Wi were the most abundant strains, occurring in 58.9 and 47.5% samples, followed by PVY NTN-NW -SYR-I (31.0%), PVY N:O (19.6%), Eu-PVY NTN (7.6%), NA-PVY N (1.3%), and PVY O (0.6%). In the 84 single-strain-infected samples, PVY N-Wi accounted for 41.7%, PVY NTN-NW -SYR-II for 40.5%, PVY NTN-NW -SYR-I for 14.3%, and PVY N:O and Eu-PVY NTN for 3.6% each. Seven isolates representing PVY NTN-NW -SYR-I (HLJ-6-1 and HLJ-9-4), PVY NTN-NW -SYR-II (INM-W-369-12 and SC-1-1-2), PVY N:O (HLJ-30-2), and PVY N-Wi (HLJ-BDH-2 and HLJ-C-429) were sequenced and analyzed molecularly. Whereas the sequence identities for isolates belonging to the same strain group were >98.5%, they fell for isolates belonging to different strain groups to 92.7–98.1% at the genome level and 96.1–98.4% at the polyprotein level. Interestingly, the exact location of the recombination events varied among isolates within a strain group. Phylogenetic analysis of all 42 full length PVY sequences from China indicated that most clustered to various recombinant groups, despite the fact that the PVY isolates were isolated from at least five host species. Pathological analysis of four isolates representing PVY N:O , PVY N-Wi , PVY NTN-NW -SYR-I, and PVY NTN-NW -SYR-II revealed that the PVY NTN-NW -SYR-II isolate incited the most severe symptoms on potato cultivar Kexin 13, followed by PVY NTN-NW -SYR-I, PVY N:O and PVY N-Wi . The PVY NTN-NW -SYR-I and PVY NTN-NW -SYR-II isolates also caused necrotic ringspots on the tubers of Kexin 13, indicating their ability to induce the potato tuber necrotic ringspot disease in potato.
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Claims 20
Claim · model-written summary
PVY was detected in 38.4% of Heilongjiang potato samples.
- Taxon
- Potato
Solanum tuberosumt0 - Interactor
- Potato virus Y
Potato virus Ye0pathogen - Outcome
- positive for Potato virus Ye0
- Value
- 38.4%e0
- Setting
- Heilongjiange0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“38.4% (Heilongjiang) and 27.7% (Inner Mongolia) were positive for Potato virus Y (PVY)”
t0“Potato virus Y in Potato Production Areas”
Claim · model-written summary
PVY was detected in 27.7% of Inner Mongolia potato samples.
- Taxon
- PotatoSolanum tuberosumt0
- Interactor
- Potato virus YPotato virus Ye0pathogen
- Outcome
- positive for Potato virus Ye0
- Value
- 27.7%e0
- Setting
- Inner Mongoliae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“38.4% (Heilongjiang) and 27.7% (Inner Mongolia) were positive for Potato virus Y (PVY)”
t0“Potato virus Y in Potato Production Areas”
Claim · model-written summary
PVY NTN-NW -SYR-II occurred in 58.9% of typed samples.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- PVY NTN-NW -SYR-IIe0
- Value
- 58.9e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PVY NTN-NW -SYR-II and PVY N-Wi were the most abundant strains, occurring in 58.9 and 47.5% samples”
t0“Potato virus Y in Potato Production Areas”
Claim · model-written summary
PVY N-Wi occurred in 47.5% of typed samples.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- PVY N-Wie0
- Value
- 47.5%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PVY NTN-NW -SYR-II and PVY N-Wi were the most abundant strains, occurring in 58.9 and 47.5% samples”
t0“Potato virus Y in Potato Production Areas”
Claim · model-written summary
PVY NTN-NW -SYR-I occurred in 31.0% of typed samples.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- PVY NTN-NW -SYR-Ie0
- Value
- 31.0%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PVY NTN-NW -SYR-I (31.0%)”
t0“Potato virus Y in Potato Production Areas”
Claim · model-written summary
PVY N:O occurred in 19.6% of typed samples.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- PVY N:Oe0
- Value
- 19.6%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PVY N:O (19.6%)”
t0“Potato virus Y in Potato Production Areas”
Claim · model-written summary
Eu-PVY NTN occurred in 7.6% of typed samples.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- Eu-PVY NTNe0
- Value
- 7.6%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Eu-PVY NTN (7.6%)”
t0“Potato virus Y in Potato Production Areas”
Claim · model-written summary
NA-PVY N occurred in 1.3% of typed samples.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- NA-PVY Ne0
- Value
- 1.3%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NA-PVY N (1.3%)”
t0“Potato virus Y in Potato Production Areas”
Claim · model-written summary
PVY O occurred in 0.6% of typed samples.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- PVY Oe0
- Value
- 0.6%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PVY O (0.6%)”
t0“Potato virus Y in Potato Production Areas”
Claim · model-written summary
Among single-strain infections, PVY N-Wi accounted for 41.7%.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- PVY N-Wie0
- Value
- 41.7%e0
- Qualifier
- 84 single-strain-infected samplese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the 84 single-strain-infected samples, PVY N-Wi accounted for 41.7%”
t0“Potato virus Y in Potato Production Areas”
Claim · model-written summary
Among single-strain infections, PVY NTN-NW -SYR-II accounted for 40.5%.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- PVY NTN-NW -SYR-IIe0
- Value
- 40.5%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PVY NTN-NW -SYR-II for 40.5%”
t0“Potato virus Y in Potato Production Areas”
Claim · model-written summary
Among single-strain infections, PVY NTN-NW -SYR-I accounted for 14.3%.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- PVY NTN-NW -SYR-Ie0
- Value
- 14.3%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PVY NTN-NW -SYR-I for 14.3%”
t0“Potato virus Y in Potato Production Areas”
Claim · model-written summary
Among single-strain infections, PVY N:O accounted for 3.6%.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- PVY N:Oe0
- Value
- 3.6%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PVY N:O and Eu-PVY NTN for 3.6% each”
t0“Potato virus Y in Potato Production Areas”
Claim · model-written summary
Among single-strain infections, Eu-PVY NTN accounted for 3.6%.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- Eu-PVY NTNe0
- Value
- 3.6%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PVY N:O and Eu-PVY NTN for 3.6% each”
t0“Potato virus Y in Potato Production Areas”
Claim · model-written summary
Within-strain PVY genome sequence identities exceeded 98.5%.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- sequence identitiese0
- Value
- >98.5%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sequence identities for isolates belonging to the same strain group were >98.5%”
t0“Potato virus Y in Potato Production Areas”
Claim · model-written summary
Between-strain PVY genome sequence identities were 92.7–98.1%.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- genome levele0
- Value
- 92.7–98.1%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolates belonging to different strain groups to 92.7–98.1% at the genome level”
t0“Potato virus Y in Potato Production Areas”
Claim · model-written summary
Between-strain PVY polyprotein sequence identities were 96.1–98.4%.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- polyprotein levele0
- Value
- 96.1–98.4%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“96.1–98.4% at the polyprotein level”
t0“Potato virus Y in Potato Production Areas”
Claim · model-written summary
Recombination locations varied among PVY isolates within a strain group.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- location of the recombination eventse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the exact location of the recombination events varied among isolates within a strain group”
t0“Potato virus Y in Potato Production Areas”
Claim · model-written summary
Most Chinese PVY sequences clustered in recombinant groups.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- clustered to various recombinant groupse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“most clustered to various recombinant groups”
t0“Potato virus Y in Potato Production Areas”
Claim · model-written summary
PVY NTN-NW -SYR-II caused the most severe symptoms on Kexin 13; both NTN-NW -SYR isolates caused tuber necrotic ringspots.
- Taxon
- potatoSolanum tuberosume0
- Material
- Kexin 13e0
- Interactor
- PVY NTN-NW -SYR-IIe0pathogen
- Outcome
- symptomse0
- Direction
- most severee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PVY NTN-NW -SYR-II isolate incited the most severe symptoms on potato cultivar Kexin 13”
e1“PVY NTN-NW -SYR-I and PVY NTN-NW -SYR-II isolates also caused necrotic ringspots on the tubers of Kexin 13”