Abstract
Multi-component analysis is one of the key techniques for the overall quality control of traditional Chinese medicines. However, the shortage and high cost of reference substances are the greatest obstacles. The substitute method is an alternative solution. In the present study, 11 compounds of rhubarb were simultaneously determined by a method named "two reference substances for determination of multiple components", which includes a qualitative method with linear calibration using two reference substances and a quantitative method with a relative correction factor combined with ultra high performance liquid chromatography. Using aloe-emodin-8-O-β-D-glucopyranoside and chrysophanol as reference compounds, chromatographic peak identification was performed. The results demonstrated that linear calibration using two reference substances method showed higher accuracy, less deviation, and better column adaptability compared to the relative retention time method. Using chrysophanol as a reference compound, the relative correction factors were determined and showed good reproducibility and stability in different laboratories with different instruments, columns, and wavelength fluctuations. The results had no significant difference compared with the external standard method. The strategy of two reference substances for determination of multiple components coupled with ultra high performance liquid chromatography is economical, efficient, accurate, reliable, and environmentally friendly and is suitable for the quality control of traditional Chinese medicines.
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Claims 4
Claim · model-written summary
The two-reference linear calibration method had higher accuracy, less deviation, and better column adaptability than relative retention time.
- Taxon
- rhubarb
Rheum rhabarbarumt0 - Outcome
- higher accuracy, less deviation, and better column adaptabilitye0
- Direction
- highere0
- Comparator
- relative retention time methode0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“linear calibration using two reference substances method showed higher accuracy, less deviation, and better column adaptability compared to the relative retention time method”
t0“rhubarb”
Claim · model-written summary
The relative correction factors were reproducible and stable across laboratories, instruments, columns, and wavelength fluctuations.
- Taxon
- rhubarbRheum rhabarbarumt0
- Outcome
- good reproducibility and stabilitye0
- Qualifier
- in different laboratories with different instruments, columns, and wavelength fluctuationse0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“relative correction factors were determined and showed good reproducibility and stability in different laboratories with different instruments, columns, and wavelength fluctuations”
t0“rhubarb”
Claim · model-written summary
Results did not differ significantly from the external standard method.
- Taxon
- rhubarbRheum rhabarbarumt0
- Outcome
- The resultse0
- Direction
- no significant differencee0
- Comparator
- external standard methode0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results had no significant difference compared with the external standard method.”
t0“rhubarb”
Claim · model-written summary
The authors conclude that the two-reference UHPLC strategy is suitable for quality control of traditional Chinese medicines.
- Taxon
- rhubarbRheum rhabarbarumt0
- Outcome
- suitable for the quality control of traditional Chinese medicinese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The strategy of two reference substances for determination of multiple components coupled with ultra high performance liquid chromatography is economical, efficient, accurate, reliable, and environmentally friendly and is suitable for the quality control of traditional Chinese medicines.”
t0“rhubarb”