Abstract
A new method of extraction by using a microwave has been widely used in the extracting of bioactive compound from plants. In this research, the pineapple (Ananas comosus L., Merr) wastes (namely skin) has been chosen as it contains a very high phenolic compound and provide a good source of antioxidant to human's health. The three parameters varied were the microwave power, the types of solvent extraction and the extraction temperature. Each of the samples was evaluated for the Total Phenolic Compound (TPC) and Antioxidant Activity (AA). The aims of this study are to obtain the optimum operating condition of Microwave-Assisted Extraction (MAE) in the extraction of phenolic compound from pineapple skins and to compare the extraction yield between MAE and Soxhlet Extraction (SE) method. From the results, it was found that the optimum condition was at 750 W microwave power, 60 C operating temperature and with the solvent ratio of ethanol: water (50-50) by volume. At this optimum condition, the TPC observed was 207.72 mg GAE/g dw, whereas for the EC 50 , DPPH value obtained was the lowest, 13.2 mg/mL. MAE has proven that this method is comparable to SE, with the TPC obtained was 28.78 mg GAE/g dw and EC 50 of 2.78 mg/L, respectively.
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