Abstract
The work reported here is an extension of studies reported in 1990. The general objective was to develop molecular markers for genotype `fingerprinting', with specific reference to possible clonal differences among `Pinot noir' clones. Leaf DNA from 8 cultivars and 9 `Pinot noir' clones were isolated. RFLP and RAPD markers were identified and used to characterize the genotypes. 65 32-P labelled cloned probes were constructed with the pUC18 plasmid and Hind-III digested `Pinot noir' DNA. The probes were tested for their ability to discriminate among the 8 cultivars. 3 probes pGAD10, pGAD15, and pGAD44 showed polymorphisms among the cultivars. pGAD15 was most useful, with 5 polymorphisms for the 8 cultivars. RAPD makers were also tested for `fingerprinting'. Several primers were tested and polymorphisms were identified among cultivars. However, significant problems with repeatability for some bands were observed. Therefore, a series of experiments were conducted to test the effect of season and extraction method. These factors did not account for the inconsistancy which seemed to be more a function of the primer used. None of these studies showed clear evidence that the `Pinot noir' clones tested were geetically different.
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Claims 6
Claim · model-written summary
Three RFLP probes showed cultivar polymorphisms.
Verbatim
e0“3 probes pGAD10, pGAD15, and pGAD44 showed polymorphisms among the cultivars.”
t0“GRAPE CULTIVARS”
Claim · model-written summary
pGAD15 was the most useful probe, showing five polymorphisms among eight cultivars.
Verbatim
e0“pGAD15 was most useful, with 5 polymorphisms for the 8 cultivars.”
t0“GRAPE CULTIVARS”
Claim · model-written summary
RAPD primers revealed polymorphisms among grape cultivars.
Verbatim
e0“Several primers were tested and polymorphisms were identified among cultivars.”
t0“GRAPE CULTIVARS”
Claim · model-written summary
Some RAPD bands had significant repeatability problems.
Verbatim
e0“significant problems with repeatability for some bands were observed.”
t0“GRAPE CULTIVARS”
Claim · model-written summary
Season and extraction method did not account for marker inconsistency, which appeared more dependent on primer.
Verbatim
e0“These factors did not account for the inconsistancy which seemed to be more a function of the primer used.”
e1“effect of season and extraction method”
t0“GRAPE CULTIVARS”
Claim · model-written summary
The tested Pinot noir clones showed no clear genetic differences.
Verbatim
e0“None of these studies showed clear evidence that the `Pinot noir' clones tested were geetically different.”