Abstract
Bacterial Panicle Blight caused by Burkholderia glumae is a major disease of rice, which has dramatically affected rice production around the world in the last years. In this study we describe the assessment of three Streptomyces isolates as biocontrol agents for B. glumae. Additionally, the presence of other plant-growth promoting abilities and their possible beneficial effects upon their inoculation on rice plants was evaluated as an ecological analysis for their future inoculation in rice crops. Two isolates (A20 and 5.1) inhibited growth of virulent B. glumae strains, as well as a wide range of bacterial and fungal species, while a third strain (7.1) showed only antifungal activity. In vitro tests demonstrated the ability of these strains to produce siderophores, Indoleacetic acid (IAA), extracellular enzymes and solubilizing phosphate. Greenhouse experiments with two rice cultivars indicated that Streptomyces A20 is able to colonize rice plants and promote plant growth in both cultivars. Furthermore, an egfp tagged mutant was generated and colonization experiments were performed, indicating that Streptomyces A20 –GFP was strongly associated with root hairs, which may be related to the plant growth promotion observed in the gnotobiotic experiments. In order to characterize the antimicrobial compounds produced by strain A20 bacteria, mass spectrometry analyses were performed. This technique indicated that A20 produced several antimicrobial compounds with sizes below 3 kDa and three of these molecules were identified as Streptotricins D, E and F. These findings indicate the potential of Streptomyces A20 as a biocontrol inoculant to protect rice plants against bacterial diseases.
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Claims 11
Claim · model-written summary
Isolates A20 and 5.1 inhibited virulent B. glumae strains.
- Taxon
- Rice
Oryza sativat0 - Material
- A20 and 5.1e0
- Interactor
- B. glumae
Burkholderia glumaee0pathogen - Outcome
- growth of virulent B. glumae strainse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two isolates (A20 and 5.1) inhibited growth of virulent B. glumae strains”
t0“Rice”
Claim · model-written summary
Isolates A20 and 5.1 also inhibited a broad range of bacteria and fungi.
- Taxon
- RiceOryza sativat0
- Material
- A20 and 5.1e0
- Interactor
- bacterial and fungal speciese0pathogen
- Outcome
- a wide range of bacterial and fungal speciese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two isolates (A20 and 5.1) inhibited growth of virulent B. glumae strains, as well as a wide range of bacterial and fungal species”
t0“Rice”
Claim · model-written summary
Isolate 7.1 showed only antifungal activity.
- Taxon
- RiceOryza sativat0
- Material
- 7.1e0
- Outcome
- only antifungal activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a third strain (7.1) showed only antifungal activity.”
t0“Rice”
Claim · model-written summary
The isolates produced siderophores, indoleacetic acid, and extracellular enzymes.
- Taxon
- RiceOryza sativat0
- Interactor
- strainse0biocontrol_agent
- Outcome
- produce siderophores, Indoleacetic acid (IAA), extracellular enzymese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these strains to produce siderophores, Indoleacetic acid (IAA), extracellular enzymes and solubilizing phosphate”
t0“Rice”
Claim · model-written summary
The isolates solubilized phosphate in vitro.
- Taxon
- RiceOryza sativat0
- Interactor
- strainse0biocontrol_agent
- Outcome
- solubilizing phosphatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these strains to produce siderophores, Indoleacetic acid (IAA), extracellular enzymes and solubilizing phosphate”
t0“Rice”
Claim · model-written summary
Streptomyces A20 colonized rice plants in the greenhouse.
- Taxon
- riceOryza sativae0
- Interactor
- Streptomyces A20e0biocontrol_agent
- Outcome
- colonize rice plantse0
- Setting
- Greenhousee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Greenhouse experiments with two rice cultivars indicated that Streptomyces A20 is able to colonize rice plants”
Claim · model-written summary
Streptomyces A20 promoted growth in both rice cultivars.
- Taxon
- riceOryza sativae0
- Treatment
- Streptomyces A20e0
- Interactor
- Streptomyces A20e0biocontrol_agent
- Outcome
- plant growthe0
- Direction
- promotee0
- Setting
- Greenhousee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Greenhouse experiments with two rice cultivars indicated that Streptomyces A20 is able to colonize rice plants and promote plant growth in both cultivars.”
Claim · model-written summary
Tagged Streptomyces A20 was strongly associated with rice root hairs.
- Taxon
- RiceOryza sativat0
- Organ / stage
- root hairse0
- Interactor
- Streptomyces A20 –GFPe0biocontrol_agent
- Outcome
- strongly associated with root hairse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Streptomyces A20 –GFP was strongly associated with root hairs”
t0“Rice”
Claim · model-written summary
A20 produced antimicrobial compounds smaller than 3 kDa.
- Taxon
- RiceOryza sativat0
- Interactor
- A20e0biocontrol_agent
- Outcome
- antimicrobial compoundse0
- Value
- below 3 kDae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A20 produced several antimicrobial compounds with sizes below 3 kDa”
t0“Rice”
Claim · model-written summary
Three antimicrobial molecules produced by A20 were identified as Streptotricins D, E and F.
- Taxon
- RiceOryza sativat0
- Outcome
- identified as Streptotricins D, E and Fe0
- Value
- threee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“three of these molecules were identified as Streptotricins D, E and F.”
t0“Rice”
Claim · model-written summary
The authors propose Streptomyces A20 as a rice biocontrol inoculant.
- Taxon
- riceOryza sativae0
- Interactor
- Streptomyces A20e0biocontrol_agent
- Outcome
- biocontrol inoculant to protect rice plants against bacterial diseasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the potential of Streptomyces A20 as a biocontrol inoculant to protect rice plants against bacterial diseases.”
The same questions in other papers
Streptomyces · growth · Oryza sativa (rice)Positive and negative results
12 papers in the same direction as this paper · 1 reporting no difference from an alternative
- positive
- positive
- no effect
10 papers more in this group
See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.