Abstract
BACKGROUND: A cryopreservation protocol has been established for oil palm somatic embryos (SEs), the efficiency of which must be evaluated, both in terms of regeneration and of long-term storage capacity, before its large-scale routine use. OBJECTIVE: To test the survival and recovery of 29 clones of oil palm somatic embryos cryostored for 20 years. MATERIALS AND METHODS: Clumps of SEs were pregrown for 7 days on medium containing 0.75 M sucrose, dehydrated in air-tight containers containing silica gel to moisture contents between 19-35% fresh weight, and then immersed directly in liquid nitrogen and stored in cryotanks for 20 years. RESULTS: Survival of SEs cryopreserved and rewarmed immediately displayed an average value of 19.1% for the 29 clones tested while survival of SEs rewarmed after 20 years of cryostorage was significantly higher, with an average of 33.2% for the 28 surviving clones. Out of these 28 surviving clones, three were lost due to contamination or regrowth decline, six produced only shoots and the rest proliferated. CONCLUSION: It is possible to cryostore oil palm SEs for extended periods and to regenerate proliferating cultures and plantlets from the cryopreserved material. The cryopreservation protocol established can thus be efficiently used to store oil palm germplasm and to manage large-scale production in industrial laboratories.
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Claims 7
Claim · model-written summary
Immediately rewarmed cryopreserved somatic embryos had mean survival of 19.1% across 29 clones.
Verbatim
e0“Survival of SEs cryopreserved and rewarmed immediately displayed an average value of 19.1% for the 29 clones tested”
t0“Oil Palm (Elaeis guineensis Jacq.)”
Claim · model-written summary
After 20 years of cryostorage, mean somatic embryo survival was significantly higher at 33.2% among 28 surviving clones.
Verbatim
e0“survival of SEs rewarmed after 20 years of cryostorage was significantly higher, with an average of 33.2% for the 28 surviving clones”
t0“Oil Palm (Elaeis guineensis Jacq.)”
Claim · model-written summary
Three of the 28 surviving clones were lost to contamination or regrowth decline.
Verbatim
e0“Out of these 28 surviving clones, three were lost due to contamination or regrowth decline”
t0“Oil Palm (Elaeis guineensis Jacq.)”
Claim · model-written summary
Six surviving clones produced only shoots.
Verbatim
e0“six produced only shoots”
t0“Oil Palm (Elaeis guineensis Jacq.)”
Claim · model-written summary
The remaining surviving clones proliferated.
Verbatim
e0“the rest proliferated”
t0“Oil Palm (Elaeis guineensis Jacq.)”
Claim · model-written summary
Extended cryostorage can regenerate proliferating cultures and plantlets from oil palm somatic embryos.
Verbatim
e0“It is possible to cryostore oil palm SEs for extended periods and to regenerate proliferating cultures and plantlets from the cryopreserved material.”
Claim · model-written summary
The authors conclude the protocol can be used to store oil palm germplasm and manage large-scale industrial production.
Verbatim
e0“The cryopreservation protocol established can thus be efficiently used to store oil palm germplasm and to manage large-scale production in industrial laboratories.”