Paper · 2019 · Ilmu Pertanian (Agricultural Science)

Genetic Variation in Selected Individuals Based on Number of Capsule in M5 Sesame Mutan Lines Detected by RAPD

Masruru Kholida; Taryono Taryono; Rani Agustina Wulandari

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citations
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Open access · cc-by-sa

Abstract

Sesame (Sesamum indicum L.) is major oilseed crops with advantages in health and food industry. Due to self pollinated crop, breeding program in sesame utilized gamma rays irradiation to increase genetic variation. The research material consisted of 164 genotypes from 22 selected individual mutant line based on the number of capsules in M5 generation. This study is to detect genetic variation in selected individuals based on number of capsules in M5 generation of sesame mutant line using RAPD markers. The analysis consists of percentage of polymorphic loci, analysis of molecular variance and visualized in cluster and co-ordinate analysis. Fifteen primers RAPD were able to amplified 237 loci. Each genotype in populations had the similarity coefficient of 0.29 – 0.85. Variance within selected individual line (66%) was higher than variance among selected mutant lines (34%). Variance in each selected individual line contributed to its high value. Line 34 showed the lowest polymorphism (23.21%) and line 19 depicted the highest polymorphism (61.60%).

Abstract from the paper’s OpenAlex record; open copy licensed cc-by-sa. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 5

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Fifteen RAPD primers amplified 237 loci.

Outcome
locie0
Value
237 locie0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sesamum indicum
Sesamum indicum · TaxonFranz Xaver · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Fifteen primers RAPD were able to amplified 237 loci.”

✓ located verbatim in the source · abstract, characters 647–700 · show in text ↑
t0

“Sesame”

✓ located verbatim in the source · title, characters 75–81 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Genotypes had similarity coefficients of 0.29–0.85.

Outcome
similarity coefficiente0
Value
0.29 – 0.85e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sesamum indicum
Sesamum indicum · TaxonFranz Xaver · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Each genotype in populations had the similarity coefficient of 0.29 – 0.85.”

✓ located verbatim in the source · abstract, characters 701–776 · show in text ↑
t0

“Sesame”

✓ located verbatim in the source · title, characters 75–81 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Variance within selected individual lines was 66%, exceeding variance among selected mutant lines.

Outcome
Variance within selected individual linee0
Direction
highere0
Value
66%e0
Qualifier
34%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sesamum indicum
Sesamum indicum · TaxonFranz Xaver · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Variance within selected individual line (66%) was higher than variance among selected mutant lines (34%).”

✓ located verbatim in the source · abstract, characters 777–883 · show in text ↑
t0

“Sesame”

✓ located verbatim in the source · title, characters 75–81 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Line 34 had the lowest polymorphism, 23.21%.

Material
Line 34e0
Outcome
polymorphisme0
Direction
loweste0
Value
23.21%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sesamum indicum
Sesamum indicum · TaxonFranz Xaver · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Line 34 showed the lowest polymorphism (23.21%)”

✓ located verbatim in the source · abstract, characters 957–1004 · show in text ↑
t0

“Sesame”

✓ located verbatim in the source · title, characters 75–81 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Line 19 had the highest polymorphism, 61.60%.

Material
line 19e0
Outcome
polymorphisme0
Direction
higheste0
Value
61.60%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sesamum indicum
Sesamum indicum · TaxonFranz Xaver · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“line 19 depicted the highest polymorphism (61.60%).”

✓ located verbatim in the source · abstract, characters 1009–1060 · show in text ↑
t0

“Sesame”

✓ located verbatim in the source · title, characters 75–81 · show in text ↑