Abstract
The aim of this study was to estimate the level of genomic and phenotypic diversity as well as the genus and species position of bacterial strains isolated from root nodules of Lembotropis nigricans (family Fabaceae). The genomic diversity of studied L. nigricans nodule symbionts was examined by using BOX-PCR and AFLP (amplified fragment length polymorphism) fingerprinting techniques. To assign bacteria to the genus, numerical analysis of phenotypic features and comparative analysis of 16S rDNA sequences were performed. The comparative analysis of combined atpD , dnaK , gyrB , and rpoB gene sequences (multilocus sequence analysis, MLSA) was used to determine the most closely related species to the studied bacteria. Both BOX-PCR and AFLP techniques revealed a high level of genomic heterogeneity of L. nigricans nodulators. Among 33 studied bacteria, 32 genotypes were delineated by the AFLP method and 27 genotypes were identified by the BOX-PCR fingerprinting. The numerical analysis of 86 phenotypic characteristics of L. nigricans nodule isolates and reference rhizobia showed that studied bacteria belong to the genus Bradyrhizobium . Affiliation of L. nigricans nodule isolates to the genus Bradyrhizobium was supported by comparative analysis of 16S rDNA sequences and the concatenation of atpD , dnaK , gyrB , and rpoB gene sequences. MLSA indicated also that L. nigricans microsymbionts are members of Bradyrhizobium japonicum . L. nigricans root nodule symbionts are members of Bradyrhizobium japonicum and exhibit high phenotypic and genomic diversity important for their survival in soil.
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Claims 5
Claim · model-written summary
BOX-PCR and AFLP fingerprinting both revealed a high level of genomic heterogeneity among L. nigricans nodulators.
- Taxon
- L. nigricans
Cytisus nigricanse0 - Interactor
- nodulatorse0symbiont
- Outcome
- high level of genomic heterogeneitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both BOX-PCR and AFLP techniques revealed a high level of genomic heterogeneity of L. nigricans nodulators”
Claim · model-written summary
Among 33 studied bacteria, AFLP delineated 32 genotypes.
- Taxon
- Lembotropis nigricansCytisus nigricanst0
- Outcome
- genotypes were delineated by the AFLP methode0
- Value
- 32 genotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among 33 studied bacteria, 32 genotypes were delineated by the AFLP method”
t0“Lembotropis nigricans”
Claim · model-written summary
Among 33 studied bacteria, BOX-PCR fingerprinting identified 27 genotypes.
- Taxon
- Lembotropis nigricansCytisus nigricanst0
- Outcome
- genotypes were identified by the BOX-PCR fingerprintinge0
- Value
- 27 genotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“27 genotypes were identified by the BOX-PCR fingerprinting”
t0“Lembotropis nigricans”
Claim · model-written summary
Numerical analysis of 86 phenotypic characteristics showed that the L. nigricans nodule isolates belong to the genus Bradyrhizobium.
- Taxon
- L. nigricansCytisus nigricanse0
- Interactor
- Bradyrhizobium
Bradyrhizobiume0symbiont - Outcome
- belong to the genus Bradyrhizobiume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The numerical analysis of 86 phenotypic characteristics of L. nigricans nodule isolates and reference rhizobia showed that studied bacteria belong to the genus Bradyrhizobium”
Claim · model-written summary
MLSA indicated that L. nigricans microsymbionts are members of Bradyrhizobium japonicum.
- Taxon
- L. nigricansCytisus nigricanse0
- Interactor
- Bradyrhizobium japonicumBradyrhizobium japonicume0symbiont
- Outcome
- members of Bradyrhizobium japonicume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MLSA indicated also that L. nigricans microsymbionts are members of Bradyrhizobium japonicum”