Abstract
The melanose disease causing Diaporthe citri is important pathogen in citrus plants worldwide. Currently, there are few researches related to biological control of melanose disease. In this study, we investigated control of Diaporthe citri by Bacillus amyloliquefaciens Y1 isolated from the soil. Low-cost fertilizer based PB medium and large-scale fermenter have been developed for field application of biological control. We prove that Y1 can be successfully grown in large-scale fermenters using PB medium without contamination. In addition, Y1 bacterial culture filtrates (BCF) of large-scale cultivation in PB medium (LP-Y1), present better growth inhibition of D. citri than small-scale cultivation in TSB medium (ST-Y1). Microscopic observations also showed that pathogen mycelial morphology was significantly affected by BCF, presumably due to metabolites produced by Y1. Finally, in pot experiment, pre-treatment with fungicide and Y1 to leaves significantly reduced disease incidence compared to the control group. This study showed that Y1 incubated with fertilizer based medium inhibited the occurrence of D. citri and could be grown in large scale field conditions without contamination as well as cost optimization.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 4
Claim · model-written summary
Y1 grew in large-scale fermenters with PB medium without contamination.
- Interactor
- Y1e0biocontrol_agent
- Outcome
- grown in large-scale fermenters using PB medium without contaminatione0
- Value
- large-scalee0
CitrusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Y1 can be successfully grown in large-scale fermenters using PB medium without contamination.”
e2“citrus plants worldwide”
Claim · model-written summary
Large-scale Y1 culture filtrate in PB medium inhibited D. citri growth more than small-scale culture in TSB medium.
- Treatment
- Y1 bacterial culture filtrates (BCF) of large-scale cultivation in PB medium (LP-Y1)e0
- Interactor
- D. citri
_Figure_4.jpg?width=60)
Diaporthe citrie0pathogen - Second interactor
- Y1e0biocontrol_agent
- Outcome
- growth inhibition of D. citrie0
- Direction
- bettere0
- Comparator
- small-scale cultivation in TSB medium (ST-Y1)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Figure_4.jpg?width=500)
Verbatim
e0“Y1 bacterial culture filtrates (BCF) of large-scale cultivation in PB medium (LP-Y1), present better growth inhibition of D. citri than small-scale cultivation in TSB medium (ST-Y1).”
e2“citrus plants worldwide”
Claim · model-written summary
Y1 culture filtrate significantly affected pathogen mycelial morphology.
- Treatment
- BCFe0
- Interactor
- pathogene0pathogen
- Second interactor
- Y1e0biocontrol_agent
- Outcome
- pathogen mycelial morphologye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pathogen mycelial morphology was significantly affected by BCF, presumably due to metabolites produced by Y1.”
e2“citrus plants worldwide”
Claim · model-written summary
Pretreatment of citrus leaves with fungicide and Y1 significantly reduced melanose incidence versus control.
- Organ / stage
- leavese0
- Treatment
- pre-treatment with fungicide and Y1e0
- Interactor
- Y1e0biocontrol_agent
- Outcome
- disease incidencee0
- Direction
- reducede0
- Comparator
- control groupe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pre-treatment with fungicide and Y1 to leaves significantly reduced disease incidence compared to the control group.”
e2“citrus plants worldwide”
The same questions in other papers
fungicide · disease · CitrusPositive and negative results
14 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
12 papers more in this group
See all 16 papers →pathogen product · Diaporthe citri · CitrusResults agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.