Abstract
Pathogen-free stocks of vegetatively propagated plants are crucial in certified plant production. They require regular monitoring of the plant germplasm for pathogens, especially of the stocks maintained in the field. Here we tested pre-basic mother plants of Fragaria, Rubus and Ribes spp., and conserved accessions of the plant genetic resources of Rubus spp. maintained at research stations in Finland, for the presence of viruses using small interfering RNA (siRNA) -based diagnostics (VirusDetect). The advance of the method is that unrelated viruses can be detected simultaneously without resumptions of the viruses present. While no virus was detected in pre-basic mother plants of Fragaria and Ribes species, rubus yellow net virus (RYNV) was detected in pre-basic mother plants of Rubus. Raspberry bushy dwarf virus (RBDV), black raspberry necrosis virus (BRNV), raspberry vein chlorosis virus (RVCV) and RYNV were detected in the Rubus genetic resource collection. The L polymerase encoding sequence characterized from seven RVCV isolates showed considerable genetic variation. The data provide the first molecular biological evidence for the presence of RYNV in Finland. RYNV was not revealed in virus indexing by indicator plants, which suggests that it may be endogenously present in some raspberry cultivars. In addition, a putative new RYNV-like badnavirus was detected in Rubus spp. Blackcurrant reversion virus (BRV) and gooseberry vein banding associated virus (GVBaV) were detected in symptomatic Ribes plants grown in the field. Results were consistent with those obtained using PCR or reverse transcription PCR and suggest that the current virus indexing methods of pre-basic mother plants work as expected. Furthermore, many new viruses were identified in the collections of plant genetic resources not previously tested for viruses. In the future, siRNA-based diagnostics could be a useful supplement for the currently used virus detection methods in certified plant production and thus rationalize and simplify the current testing system.
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Claims 16
Claim · model-written summary
no virus was detected in Fragaria.
- Outcome
- no virus was detectede0
.jpg?width=60){kind=small}
FragariaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“no virus was detected in pre-basic mother plants of Fragaria and Ribes species”
Claim · model-written summary
no virus was detected in Ribes.
- Outcome
- no virus was detectede0
RibesOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no virus was detected in pre-basic mother plants of Fragaria and Ribes species”
Claim · model-written summary
rubus yellow net virus (RYNV) was detected in Rubus.
- Interactor
- rubus yellow net virus (RYNV)Rubus yellow net viruse0pathogen
- Outcome
- rubus yellow net virus (RYNV) was detectede0
RubusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rubus yellow net virus (RYNV) was detected in pre-basic mother plants of Rubus”
Claim · model-written summary
Raspberry bushy dwarf virus (RBDV) in Rubus.
- Interactor
- Raspberry bushy dwarf virus (RBDV)Raspberry bushy dwarf viruse0pathogen
- Outcome
- Raspberry bushy dwarf virus (RBDV)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Raspberry bushy dwarf virus (RBDV), black raspberry necrosis virus (BRNV), raspberry vein chlorosis virus (RVCV) and RYNV were detected in the Rubus genetic resource collection”
Claim · model-written summary
black raspberry necrosis virus (BRNV) in Rubus.
- Interactor
- black raspberry necrosis virus (BRNV)Black raspberry necrosis viruse0pathogen
- Outcome
- black raspberry necrosis virus (BRNV)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Raspberry bushy dwarf virus (RBDV), black raspberry necrosis virus (BRNV), raspberry vein chlorosis virus (RVCV) and RYNV were detected in the Rubus genetic resource collection”
Claim · model-written summary
raspberry vein chlorosis virus (RVCV) in Rubus.
- Interactor
- raspberry vein chlorosis virus (RVCV)
.jpg?width=60)
Raspberry vein chlorosis viruse0pathogen - Outcome
- raspberry vein chlorosis virus (RVCV)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Raspberry bushy dwarf virus (RBDV), black raspberry necrosis virus (BRNV), raspberry vein chlorosis virus (RVCV) and RYNV were detected in the Rubus genetic resource collection”
Claim · model-written summary
RYNV were detected in Rubus.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Raspberry bushy dwarf virus (RBDV), black raspberry necrosis virus (BRNV), raspberry vein chlorosis virus (RVCV) and RYNV were detected in the Rubus genetic resource collection”
Claim · model-written summary
RVCV L polymerase sequences from seven isolates had considerable variation.
- Interactor
- RVCVe0pathogen
- Gene
- L polymerase encoding sequencee0
- Outcome
- considerable genetic variatione0
- Value
- seven RVCV isolatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The L polymerase encoding sequence characterized from seven RVCV isolates showed considerable genetic variation”
t0“plant genetic resources of Rubus spp. maintained”
Claim · model-written summary
Molecular evidence established RYNV presence in Finland.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the first molecular biological evidence for the presence of RYNV in Finland”
t0“plant genetic resources of Rubus spp. maintained”
Claim · model-written summary
Indicator-plant indexing did not reveal RYNV.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RYNV was not revealed in virus indexing by indicator plants”
t0“plant genetic resources of Rubus spp. maintained”
Claim · model-written summary
A putative new RYNV-like badnavirus was detected in Rubus.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a putative new RYNV-like badnavirus was detected in Rubus spp.”
Claim · model-written summary
Blackcurrant reversion virus (BRV) in Ribes.
- Interactor
- Blackcurrant reversion virus (BRV)Blackcurrant reversion viruse0pathogen
- Outcome
- Blackcurrant reversion virus (BRV)e0
- Setting
- fielde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Blackcurrant reversion virus (BRV) and gooseberry vein banding associated virus (GVBaV) were detected in symptomatic Ribes plants grown in the field”
Claim · model-written summary
gooseberry vein banding associated virus (GVBaV) were detected in Ribes.
- Interactor
- gooseberry vein banding associated virus (GVBaV)Gooseberry vein banding associated viruse0pathogen
- Outcome
- gooseberry vein banding associated virus (GVBaV) were detectede0
- Setting
- fielde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Blackcurrant reversion virus (BRV) and gooseberry vein banding associated virus (GVBaV) were detected in symptomatic Ribes plants grown in the field”
Claim · model-written summary
Virus detection results agreed with PCR and reverse transcription PCR.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Results were consistent with those obtained using PCR or reverse transcription PCR”
t0“plant genetic resources of Rubus spp. maintained”
Claim · model-written summary
Many new viruses were found in previously untested plant genetic resource collections.
- Outcome
- many new viruses were identifiede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“many new viruses were identified in the collections of plant genetic resources not previously tested for viruses”
t0“plant genetic resources of Rubus spp. maintained”
Claim · model-written summary
Authors suggest siRNA diagnostics could supplement existing virus detection in certified plant production.
- Outcome
- siRNA-based diagnostics could be a useful supplemente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“siRNA-based diagnostics could be a useful supplement for the currently used virus detection methods in certified plant production”
t0“plant genetic resources of Rubus spp. maintained”