Abstract
Cultivated lentil (Lens culinaris Medik.) is one of the oldest domesticated crops and one of the most important grain legumes worldwide. The Mediterranean Basin holds large part of lentil biodiversity, however no genetic structure was defined within the Mediterranean gene pool. In this study, we used high-throughput genotyping by sequencing (GBS) to resolve the genetic structure of the Mediterranean ex situ lentil collection held at the Italian National Research Council. Sequencing of a 188-plex GBS library and bioinformatics treatment of data yielded 6,693 single nucleotide polymorphisms (SNPs). Analysis of non-redundant genotypes with non-parametric and parametric methods highlighted the occurrence of five highly differentiated genetic clusters. Clustering could be related to geographic patterns and phenotypic traits, indicating that post-domestication routes introducing cultivation in Mediterranean countries and selection were major forces shaping lentil population structure. The estimation of the fixation index FST at individual SNP loci allowed the identification of distinctive alleles across clusters, suggesting the possibility to set-up molecular keys for the assignment of lentil germplasm to specific genetic groups. Finally, significant associations between markers and phenotypic data were identified. Overall, the results of this study are of major importance for lentil conservation genetics and breeding and provide insights on the lentil evolutionary history.
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Claims 7
Claim · model-written summary
GBS yielded 6,693 SNPs for the Mediterranean lentil collection.
- Taxon
- Lens culinaris
Lens culinarist0 - Outcome
- single nucleotide polymorphismse1
- Value
- 6,693e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Lens culinaris”
e1“Sequencing of a 188-plex GBS library and bioinformatics treatment of data yielded 6,693 single nucleotide polymorphisms (SNPs)”
Claim · model-written summary
Non-redundant lentil genotypes formed five highly differentiated genetic clusters.
- Taxon
- Lens culinarisLens culinarist0
- Outcome
- genetic clusterse2
- Value
- fivee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Lens culinaris”
e2“Analysis of non-redundant genotypes with non-parametric and parametric methods highlighted the occurrence of five highly differentiated genetic clusters”
Claim · model-written summary
Lentil genetic clustering could be related to geographic patterns and phenotypic traits.
- Taxon
- Lens culinarisLens culinarist0
- Outcome
- Clusteringe3
- Setting
- geographic patternse3
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Lens culinaris”
e3“Clustering could be related to geographic patterns and phenotypic traits”
Claim · model-written summary
Post-domestication routes introducing cultivation in Mediterranean countries and selection were major forces shaping lentil population structure.
- Taxon
- Lens culinarisLens culinarist0
- Outcome
- population structuree3
- Qualifier
- post-domestication routes introducing cultivation in Mediterranean countries and selectione3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Lens culinaris”
e3“indicating that post-domestication routes introducing cultivation in Mediterranean countries and selection were major forces shaping lentil population structure”
Claim · model-written summary
FST at individual SNP loci identified distinctive alleles across lentil clusters.
- Taxon
- Lens culinarisLens culinarist0
- Gene
- individual SNP locie4
- Outcome
- distinctive allelese4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Lens culinaris”
e4“The estimation of the fixation index FST at individual SNP loci allowed the identification of distinctive alleles across clusters”
Claim · model-written summary
Distinctive alleles may allow molecular keys for assigning lentil germplasm to specific genetic groups.
- Taxon
- Lens culinarisLens culinarist0
- Outcome
- molecular keys for the assignment of lentil germplasm to specific genetic groupse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Lens culinaris”
e4“suggesting the possibility to set-up molecular keys for the assignment of lentil germplasm to specific genetic groups”
Claim · model-written summary
Significant associations were identified between lentil markers and phenotypic data.
- Taxon
- Lens culinarisLens culinarist0
- Gene
- markerse5
- Outcome
- associations between markers and phenotypic datae5
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Lens culinaris”
e5“Finally, significant associations between markers and phenotypic data were identified”