Abstract
A simplest, rapid, reliable, and cost-effective staining method of the hypocotyls in the infected lupine plants was modified from Woodbury method for Native PAGE catalase to define the role of the fungal catalase produced by F. oxysporum on the reactive oxygen species (ROS) of Lupinus termis during infection. Fresh sections in hypocotyls of the infected plant and control ones were taken and stained directly on a glass slide. The visual detection of oxygen evolution from the xylem vessel, as a fungal catalase activity, gives a potential evidence for its role in embolism formation and wilt disease. White and blue color indicates the presence of catalase and H2O2 respectively. The catalase activity was significantly increased in the fungal growth medium than in the control plants. A model has built up to summarize the role of fungal catalase in the embolism formation, water current disconnection, and wilt disease.
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Claims 6
Claim · model-written summary
The modified staining method is described as rapid, reliable and cost-effective for infected lupine hypocotyls.
Verbatim
e0“A simplest, rapid, reliable, and cost-effective staining method of the hypocotyls in the infected lupine plants was modified from Woodbury method for Native PAGE catalase”
Claim · model-written summary
Oxygen evolution detected in xylem vessels provides potential evidence for fungal catalase involvement in embolism and wilt.
- Taxon
- Lupinus termis
Lupinus albuse1 - Organ / stage
- xylem vessele0
- Interactor
- F. oxysporum
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=60)
Fusarium oxysporume1pathogen - Gene
- fungal catalasee1
- Outcome
- embolism formation and wilt diseasee0
- Qualifier
- potential evidencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=500)
Verbatim
e0“The visual detection of oxygen evolution from the xylem vessel, as a fungal catalase activity, gives a potential evidence for its role in embolism formation and wilt disease.”
e1“fungal catalase produced by F. oxysporum on the reactive oxygen species (ROS) of Lupinus termis during infection”
Claim · model-written summary
White staining indicates catalase presence.
- Taxon
- Lupinus termisLupinus albuse1
- Outcome
- presence of catalasee0
- Value
- Whitee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“White and blue color indicates the presence of catalase and H2O2 respectively.”
e1“Lupinus termis”
Claim · model-written summary
Blue staining indicates H2O2 presence.
- Taxon
- Lupinus termisLupinus albuse1
- Outcome
- H2O2e0
- Value
- bluee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“White and blue color indicates the presence of catalase and H2O2 respectively.”
e1“Lupinus termis”
Claim · model-written summary
Catalase activity was significantly higher in fungal growth medium than in control plants.
- Taxon
- F. oxysporumFusarium oxysporume1
- Outcome
- catalase activitye0
- Direction
- increasede0
- Comparator
- control plantse0
- Setting
- fungal growth mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The catalase activity was significantly increased in the fungal growth medium than in the control plants.”
e1“fungal catalase produced by F. oxysporum”
Claim · model-written summary
A model was built summarizing fungal catalase involvement in embolism, water current disconnection and wilt.
- Taxon
- Lupinus termisLupinus albuse1
- Gene
- fungal catalasee0
- Outcome
- modele0
- Qualifier
- embolism formation, water current disconnection, and wilt diseasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A model has built up to summarize the role of fungal catalase in the embolism formation, water current disconnection, and wilt disease.”
e1“Lupinus termis”