Abstract
The nifV gene encodes homocitrate synthase, the enzyme that catalyzes the formation of homocitrate, which is essential for arranging the FeMo-cofactor in the catalytic center of nitrogenase. Some host plants, such as Lotus japonicus, supply homocitrate to their symbionts, in this case, Mesorhizobium loti, which lacks nifV. In contrast, Bradyrhizobium ORS285, a symbiont of Aeschynomene cross-inoculation (CI) groups 2 and 3, requires nifV for symbiosis with Aeschynomene species that belong to CI group 3, and some species belonging to CI group 2. However, it currently remains unclear whether rhizobial nifV is required for symbiosis with Aeschynomene species belonging to CI group 1 or with other legumes. We generated nifV-disruption (ΔnifV) mutants of two wide-host-range rhizobia, Bradyrhizobium SUTN9-2 and DOA9, to investigate whether they require nifV for symbiosis. Both ΔnifV mutant strains showed significantly less nitrogenase activity in a free-living state than the respective wild-type strains. The symbiotic phenotypes of SUTN9-2, DOA9, and their ΔnifV mutants were examined with four legumes, Aeschynomene americana, Stylosanthes hamata, Indigofera tinctoria, and Desmodium tortuosum. nifV was required for the efficient symbiosis of SUTN9-2 with A. americana (CI group 1), but not for that of DOA9. SUTN9-2 established symbiosis with all three other legumes; nifV was required for symbiosis with I. tinctoria and D. tortuosum. These results suggest that, in addition to Aeschynomene CI groups 2 and 3, CI group 1 and several other legumes require the rhizobial nifV for symbiosis.
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Claims 5
Claim · model-written summary
SUTN9-2 nifV was required for efficient symbiosis with Aeschynomene americana.
- Taxon
- A. americana
Aeschynomene americanae0 - Treatment
- SUTN9-2e0
- Interactor
- SUTN9-2e0symbiont
- Gene
- nifVe0
- Outcome
- efficient symbiosise0
- Direction
- requirede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nifV was required for the efficient symbiosis of SUTN9-2 with A. americana (CI group 1)”
Claim · model-written summary
DOA9 nifV was not required for efficient symbiosis with Aeschynomene americana.
- Taxon
- A. americanaAeschynomene americanae1
- Interactor
- DOA9e0symbiont
- Gene
- nifVe0
- Outcome
- symbiosise0
- Direction
- note0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nifV was required for the efficient symbiosis of SUTN9-2 with A. americana (CI group 1), but not for that of DOA9”
e1“with A. americana (CI group 1), but not for that of DOA9”
Claim · model-written summary
SUTN9-2 established symbiosis with the three other tested legumes.
Verbatim
e0“SUTN9-2 established symbiosis with all three other legumes”
Claim · model-written summary
SUTN9-2 nifV was required for symbiosis with Indigofera tinctoria.
- Taxon
- I. tinctoria
Indigofera tinctoriae0 - Gene
- nifVe0
- Outcome
- symbiosise0
- Direction
- requirede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nifV was required for symbiosis with I. tinctoria”
Claim · model-written summary
SUTN9-2 nifV was required for symbiosis with Desmodium tortuosum.
- Taxon
- D. tortuosum
Desmodium tortuosume0 - Gene
- nifVe0
- Outcome
- symbiosise0
- Direction
- requirede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nifV was required for symbiosis with I. tinctoria and D. tortuosum”