Abstract
AIMS: This study aimed to obtain an antagonistic endophyte against Sclerotium rolfsii from peanut seeds, evaluate the biocontrol efficacy towards peanut stem rot and explore its antifungal mechanism against S. rolfsii. METHODS AND RESULTS: Thirty-seven endophytic bacteria were isolated from peanut seeds, six of which exhibited stronger antagonistic activities against S. rolfsii (inhibition rate, IR of hyphae growth ≥70%). Strain LHSB1, the strongest antagonistic strain, was identified as Bacillus velezensis. LHSB1 showed 93·8% of radial growth inhibition of S. rolfsii hyphae and exhibited obvious antagonistic activity against another six pathogenic fungi of peanut. Pot experiments showed two different LHSB1 treatments both significantly reduced the disease incidence and severity of stem rot (P < 0·05) compared to the controls, and the biocontrol efficacy reached 62·6-70·8%, significantly higher than that of Carbendazim control (P < 0·05). Further analyses revealed LHSB1 culture filtrate significantly inhibited sclerotia formation and germination, caused the abnormalities and membrane integrity damage of S. rolfsii hyphae, which might be the possible mode of action of LHSB1 against S. rolfsii. Three antifungal lipopeptides bacillomycin A, surfactin A and fengycin A, were detected in LHSB1 culture extracts by UPLC-ESI-MS, which could be responsible for the biocontrol activity of LHSB1 against S. rolfsii. CONCLUSION: Our results suggested that the seed-borne endophytic B. velezensis LHSB1 would be a tremendous potential agent for the biocontrol of peanut stem rot caused by S. rolfsii. SIGNIFICANCE AND IMPACT OF THE STUDY: This comprehensive study provides a candidate endophytic biocontrol strain and reveals its antifungal mechanism against S. rolfsi. To the best of our knowledge, this is the first time that seed-borne endophytic B. velezensis was used as the biocontrol agent to control peanut stem rot.
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Claims 11
Claim · model-written summary
Six seed endophytes inhibited S. rolfsii hyphal growth by at least 70%.
- Taxon
- peanut
Arachis hypogaeat0 - Interactor
- S. rolfsii
Agroathelia rolfsiie0pathogen - Outcome
- inhibition rate, IR of hyphae growthe0
- Value
- ≥70%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“six of which exhibited stronger antagonistic activities against S. rolfsii (inhibition rate, IR of hyphae growth ≥70%)”
t0“peanut”
Claim · model-written summary
The strongest antagonistic isolate LHSB1 was identified as Bacillus velezensis.
- Taxon
- peanutArachis hypogaeat0
- Interactor
- Bacillus velezensis
Bacillus velezensise0biocontrol_agent - Outcome
- identified as Bacillus velezensise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Strain LHSB1, the strongest antagonistic strain, was identified as Bacillus velezensis”
t0“peanut”
Claim · model-written summary
LHSB1 inhibited S. rolfsii hyphal radial growth by 93·8%.
- Taxon
- peanutArachis hypogaeat0
- Interactor
- S. rolfsiiAgroathelia rolfsiie0pathogen
- Outcome
- radial growth inhibition of S. rolfsii hyphaee0
- Value
- 93·8%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LHSB1 showed 93·8% of radial growth inhibition of S. rolfsii hyphae”
t0“peanut”
Claim · model-written summary
LHSB1 antagonized another six pathogenic fungi of peanut.
- Taxon
- peanutArachis hypogaeae0
- Interactor
- pathogenic fungie0pathogen
- Outcome
- antagonistic activitye0
- Value
- six pathogenic fungie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exhibited obvious antagonistic activity against another six pathogenic fungi of peanut”
Claim · model-written summary
Both LHSB1 treatments significantly reduced stem rot incidence relative to controls.
- Taxon
- peanutArachis hypogaeat0
- Treatment
- LHSB1 treatmentse0
- Interactor
- Sclerotium rolfsiiAgroathelia rolfsiit0pathogene0
- Second interactor
- LHSB1e0biocontrol_agent
- Outcome
- disease incidencee0
- Direction
- reducede0
- Comparator
- controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two different LHSB1 treatments both significantly reduced the disease incidence and severity of stem rot (P < 0·05) compared to the controls”
t0“Seed‐borne endophytic Bacillus velezensis LHSB1 mediate the biocontrol of peanut stem rot caused by Sclerotium rolfsii”
Claim · model-written summary
Both LHSB1 treatments significantly reduced stem rot severity relative to controls.
- Taxon
- peanutArachis hypogaeat0
- Treatment
- LHSB1 treatmentse0
- Interactor
- Sclerotium rolfsiiAgroathelia rolfsiit0pathogene0
- Second interactor
- LHSB1e0biocontrol_agent
- Outcome
- severity of stem rote0
- Direction
- reducede0
- Comparator
- controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two different LHSB1 treatments both significantly reduced the disease incidence and severity of stem rot (P < 0·05) compared to the controls”
t0“Seed‐borne endophytic Bacillus velezensis LHSB1 mediate the biocontrol of peanut stem rot caused by Sclerotium rolfsii”
Claim · model-written summary
LHSB1 biocontrol efficacy reached 62·6-70·8%, significantly above Carbendazim.
- Taxon
- peanutArachis hypogaeat0
- Treatment
- LHSB1t0
- Interactor
- Sclerotium rolfsiiAgroathelia rolfsiit0pathogene0
- Outcome
- biocontrol efficacye0
- Direction
- highere0
- Value
- 62·6-70·8%e0
- Comparator
- Carbendazim controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“biocontrol efficacy reached 62·6-70·8%, significantly higher than that of Carbendazim control (P < 0·05)”
t0“Seed‐borne endophytic Bacillus velezensis LHSB1 mediate the biocontrol of peanut stem rot caused by Sclerotium rolfsii”
Claim · model-written summary
LHSB1 culture filtrate significantly inhibited sclerotial formation and germination.
- Taxon
- peanutArachis hypogaeat0
- Treatment
- LHSB1 culture filtratee0
- Interactor
- S. rolfsiiAgroathelia rolfsiie0pathogen
- Outcome
- sclerotia formation and germinatione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LHSB1 culture filtrate significantly inhibited sclerotia formation and germination, caused the abnormalities and membrane integrity damage of S. rolfsii hyphae”
t0“peanut”
Claim · model-written summary
LHSB1 culture filtrate caused S. rolfsii hyphal abnormalities and membrane damage.
- Taxon
- peanutArachis hypogaeat0
- Treatment
- LHSB1 culture filtratee0
- Interactor
- S. rolfsiiAgroathelia rolfsiie0pathogen
- Outcome
- abnormalities and membrane integrity damage of S. rolfsii hyphaee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LHSB1 culture filtrate significantly inhibited sclerotia formation and germination, caused the abnormalities and membrane integrity damage of S. rolfsii hyphae”
t0“peanut”
Claim · model-written summary
Bacillomycin A, surfactin A and fengycin A were detected in LHSB1 extracts.
- Taxon
- peanutArachis hypogaeat0
- Interactor
- LHSB1e0biocontrol_agent
- Outcome
- bacillomycin A, surfactin A and fengycin Ae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three antifungal lipopeptides bacillomycin A, surfactin A and fengycin A, were detected in LHSB1 culture extracts by UPLC-ESI-MS”
t0“peanut”
Claim · model-written summary
The authors suggest LHSB1 as a potential biocontrol agent for peanut stem rot.
- Taxon
- peanutArachis hypogaeae0
- Interactor
- S. rolfsiiAgroathelia rolfsiie0pathogen
- Second interactor
- B. velezensis LHSB1Bacillus velezensise0biocontrol_agent
- Outcome
- biocontrol of peanut stem rote0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“seed-borne endophytic B. velezensis LHSB1 would be a tremendous potential agent for the biocontrol of peanut stem rot caused by S. rolfsii”
The same questions in other papers
pathogen product · Agroathelia rolfsii · Arachis hypogaea (peanut)Positive and negative results
4 papers in the same direction as this paper · 2 in the opposite direction
- positive
- no effect
- negative
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.