Paper · 2019 · Comparative Cytogenetics

Molecular-cytogenetic analysis of diploid wheatgrass Thinopyrum bessarabicum (Savul. and Rayss) A. Löve

Ekaterina D. Badaeva; Sergei A. Surzhikov; Alexander V. Agafonov

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claims
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verbatim quotes
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citations
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research areas
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Open access · cc-by

Abstract

Thinopyrum bessarabicum (T. Săvulescu & T. Rayss, 1923) A. Löve, 1980 is diploid (2n=2x=14, JJ or E b E b ), perennial self-fertilizing rhizomatous maritime beach grass, which is phylogenetically close to another diploid wheatgrass species, Agropyron elongatum (N. Host, 1797) P. de Beauvois, 1812. The detailed karyotype of Th. bessarabicum was constructed based on FISH with six DNA probes representing 5S and 45S rRNA gene families and four tandem repeats. We found that the combination of pAesp_SAT86 (= pTa-713) probe with pSc119.2 or pAs1/ pTa-535 allows the precise identification of all J-genome chromosomes. Comparison of our data with the results of other authors showed that karyotypically Th. bessarabicum is distinct from A. elongatum . On the other hand, differences between the J-genome chromosomes of Th. bessarabicum and the chromosomes of hexaploid Th. intermedium (N. Host, 1797) M. Barkworth & D.R. Dewey, 1985 and decaploid Th. ponticum (J. Podpěra, 1902) Z.–W. Liu & R.–C. Wang, 1993 in the distribution of rDNA loci and hybridization patterns of pSc119.2 and pAs1 probes could be an indicative of (1) this diploid species was probably not involved in the origin of these polyploids or (2) it could has contributed the J-genome to Th. intermedium and Th. ponticum , but it was substantially modified over the course of speciation

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Claims 4

Observationobservedoriginal resultmeasured

Claim · model-written summary

The combination of pAesp_SAT86 (= pTa-713) probe with pSc119.2 or pAs1/ pTa-535 allows precise identification of all J-genome chromosomes.

Taxon
Thinopyrum bessarabicumThinopyrum bessarabicumt0
Treatment
pAesp_SAT86 (= pTa-713) probee1
Outcome
precise identification of all J-genome chromosomese1

Verbatim

t0

“Thinopyrum bessarabicum”

✓ located verbatim in the source · title, characters 53–76 · show in text ↑
e1

“the combination of pAesp_SAT86 (= pTa-713) probe with pSc119.2 or pAs1/ pTa-535 allows the precise identification of all J-genome chromosomes”

✓ located verbatim in the source · abstract, characters 474–615 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The detailed karyotype of Thinopyrum bessarabicum was constructed by FISH with six DNA probes.

Taxon
Th. bessarabicume0
Treatment
FISH with six DNA probese0
Outcome
detailed karyotypee0

Verbatim

t0

“Thinopyrum bessarabicum”

✓ located verbatim in the source · title, characters 53–76 · show in text ↑
e0

“The detailed karyotype of Th. bessarabicum was constructed based on FISH with six DNA probes representing 5S and 45S rRNA gene families and four tandem repeats”

✓ located verbatim in the source · abstract, characters 299–458 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Thinopyrum bessarabicum is karyotypically distinct from Agropyron elongatum.

Taxon
Th. bessarabicume2
Outcome
karyotypically Th. bessarabicum is distincte2
Comparator
A. elongatume2

Verbatim

t0

“Thinopyrum bessarabicum”

✓ located verbatim in the source · title, characters 53–76 · show in text ↑
e2

“Comparison of our data with the results of other authors showed that karyotypically Th. bessarabicum is distinct from A. elongatum”

✓ located verbatim in the source · abstract, characters 617–747 · show in text ↑
Observationhypothesisoriginal resultmeasured

Claim · model-written summary

Differences in rDNA loci and hybridization patterns of pSc119.2 and pAs1 probes may indicate that the diploid species was not involved in the origin of polyploid Thinopyrum species or was modified during speciation.

Taxon
Thinopyrum bessarabicumThinopyrum bessarabicumt0
Outcome
distribution of rDNA loci and hybridization patterns of pSc119.2 and pAs1 probese3

Verbatim

t0

“Thinopyrum bessarabicum”

✓ located verbatim in the source · title, characters 53–76 · show in text ↑
e3

“in the distribution of rDNA loci and hybridization patterns of pSc119.2 and pAs1 probes could be an indicative of”

✓ located verbatim in the source · abstract, characters 1006–1119 · show in text ↑