Abstract
The symbiont endophytic fungi in tobacco are highly diverse and difficult to classify. Here, we sequenced the genomes of Curvularia trifolii and Leptosphaerulina chartarum isolated from tobacco plants. Finally, 41.68 Mb and 37.95 Mb nuclear genomes were sequenced for C. trifolii and L. chartarum with the scaffold N50, accounting for 638.94 Kb and 284.12 Kb, respectively. Meanwhile, we obtained 68,926 bp and 59,100 bp for their mitochondrial genomes. To more accurately classify C. trifolii and L. chartarum, we extracted seven nuclear genes and 12 mitochondrial genes from these two genomes and their closely related species. The genes were then used for calculation of evolutionary rates and for phylogenetic analysis. Results showed that it was difficult to achieve consistent results using a single gene due to their different evolutionary rates, while the phylogenetic trees obtained by combining datasets showed stable topologies. It is, therefore, more accurate to construct phylogenetic relationships for endophytic fungi based on multi-gene datasets. This study provides new insights into the distribution and characteristics of endophytic fungi in tobacco.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
The nuclear genome of Curvularia trifolii was 41.68 Mb.
- Taxon
- tobacco
Nicotiana tabacume0 - Interactor
- C. trifoliiCurvularia trifoliie2symbiont
- Outcome
- nuclear genomese2
- Value
- 41.68 Mbe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The symbiont endophytic fungi in tobacco are highly diverse and difficult to classify”
e2“Finally, 41.68 Mb and 37.95 Mb nuclear genomes were sequenced for C. trifolii and L. chartarum with the scaffold N50, accounting for 638.94 Kb and 284.12 Kb, respectively”
Claim · model-written summary
The nuclear genome of Leptosphaerulina chartarum was 37.95 Mb.
- Taxon
- tobaccoNicotiana tabacume0
- Interactor
- L. chartarumPseudopithomyces chartarume2symbiont
- Outcome
- nuclear genomese2
- Value
- 37.95 Mbe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The symbiont endophytic fungi in tobacco are highly diverse and difficult to classify”
e2“Finally, 41.68 Mb and 37.95 Mb nuclear genomes were sequenced for C. trifolii and L. chartarum with the scaffold N50, accounting for 638.94 Kb and 284.12 Kb, respectively”
Claim · model-written summary
The scaffold N50 of Curvularia trifolii was 638.94 Kb.
- Taxon
- tobaccoNicotiana tabacume0
- Interactor
- C. trifoliiCurvularia trifoliie2symbiont
- Outcome
- scaffold N50e2
- Value
- 638.94 Kbe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The symbiont endophytic fungi in tobacco are highly diverse and difficult to classify”
e2“Finally, 41.68 Mb and 37.95 Mb nuclear genomes were sequenced for C. trifolii and L. chartarum with the scaffold N50, accounting for 638.94 Kb and 284.12 Kb, respectively”
Claim · model-written summary
The scaffold N50 of Leptosphaerulina chartarum was 284.12 Kb.
- Taxon
- tobaccoNicotiana tabacume0
- Interactor
- L. chartarumPseudopithomyces chartarume2symbiont
- Outcome
- scaffold N50e2
- Value
- 284.12 Kbe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The symbiont endophytic fungi in tobacco are highly diverse and difficult to classify”
e2“Finally, 41.68 Mb and 37.95 Mb nuclear genomes were sequenced for C. trifolii and L. chartarum with the scaffold N50, accounting for 638.94 Kb and 284.12 Kb, respectively”
Claim · model-written summary
The mitochondrial genome of Curvularia trifolii was 68,926 bp.
- Taxon
- tobaccoNicotiana tabacume0
- Interactor
- Curvularia trifoliiCurvularia trifoliie1symbiont
- Outcome
- mitochondrial genomese3
- Value
- 68,926 bpe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The symbiont endophytic fungi in tobacco are highly diverse and difficult to classify”
e1“we sequenced the genomes of Curvularia trifolii and Leptosphaerulina chartarum isolated from tobacco plants”
e3“Meanwhile, we obtained 68,926 bp and 59,100 bp for their mitochondrial genomes”
Claim · model-written summary
The mitochondrial genome of Leptosphaerulina chartarum was 59,100 bp.
- Taxon
- tobaccoNicotiana tabacume0
- Interactor
- Leptosphaerulina chartarumPseudopithomyces chartarume1symbiont
- Outcome
- mitochondrial genomese3
- Value
- 59,100 bpe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The symbiont endophytic fungi in tobacco are highly diverse and difficult to classify”
e1“we sequenced the genomes of Curvularia trifolii and Leptosphaerulina chartarum isolated from tobacco plants”
e3“Meanwhile, we obtained 68,926 bp and 59,100 bp for their mitochondrial genomes”
Claim · model-written summary
A single gene gave inconsistent phylogenetic results for these endophytic fungi because of different evolutionary rates.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- consistent resultse4
- Comparator
- single genee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The symbiont endophytic fungi in tobacco are highly diverse and difficult to classify”
e4“it was difficult to achieve consistent results using a single gene due to their different evolutionary rates”
Claim · model-written summary
Phylogenetic trees from combined datasets showed stable topologies.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- stable topologiese5
- Comparator
- combining datasetse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The symbiont endophytic fungi in tobacco are highly diverse and difficult to classify”
e5“the phylogenetic trees obtained by combining datasets showed stable topologies”
Claim · model-written summary
Phylogenetic relationships of endophytic fungi are more accurately constructed from multi-gene datasets.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- multi-gene datasetse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The symbiont endophytic fungi in tobacco are highly diverse and difficult to classify”
e6“It is, therefore, more accurate to construct phylogenetic relationships for endophytic fungi based on multi-gene datasets”